Cytokine · 2025
Effects of acute heat exposure on monocyte phenotype following exhaustive exercise: An ex vivo study.
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de Lima Schipper Lucas, da Silva Igor Martins, Marmett Bruna, Teixeira Paula Coelho, de Lira Fabio Santos, Dorneles Gilson Pires, Romão Pedro R T
Аннотация
BACKGROUND/AIM: This study aimed to evaluate the effects of ex vivo whole blood heat therapy on monocyte inflammatory response following exhaustive exercise in young individuals.
METHODS: Eight recreationally endurance trained individuals (27.2 ± 3.1 years old, VO₂Peak 53.1 ± 2.1 mL/kg/min) performed a strenuous step-up/step-down protocol until exhaustion. Blood samples were collected pre and post exercise and incubated 37 °C or 40 °C during 2 h. Flow cytometry was used to assess TLR-4 expression, NF-kB activation, mitochondrial membrane potential and TNF-alpha expression in CD14+ monocytes.
RESULTS: Post exercise, TLR-4 expression and NF-kB activation increased at 37 °C (p < 0.001), but heat therapy at 40 °C significantly reduced these responses (p < 0.01). MMP decreased after exercise at both temperatures but was significantly lower at 37 °C than 40 °C (p < 0.05). TNF-a levels showed a trend toward returning to baseline with heat therapy.
CONCLUSION: Ex vivo heat therapy reduces monocyte mediated inflammation after exercise, induced muscle damage, suggesting its potential as a recovery strategy to strenuous exercise sessions.
METHODS: Eight recreationally endurance trained individuals (27.2 ± 3.1 years old, VO₂Peak 53.1 ± 2.1 mL/kg/min) performed a strenuous step-up/step-down protocol until exhaustion. Blood samples were collected pre and post exercise and incubated 37 °C or 40 °C during 2 h. Flow cytometry was used to assess TLR-4 expression, NF-kB activation, mitochondrial membrane potential and TNF-alpha expression in CD14+ monocytes.
RESULTS: Post exercise, TLR-4 expression and NF-kB activation increased at 37 °C (p < 0.001), but heat therapy at 40 °C significantly reduced these responses (p < 0.01). MMP decreased after exercise at both temperatures but was significantly lower at 37 °C than 40 °C (p < 0.05). TNF-a levels showed a trend toward returning to baseline with heat therapy.
CONCLUSION: Ex vivo heat therapy reduces monocyte mediated inflammation after exercise, induced muscle damage, suggesting its potential as a recovery strategy to strenuous exercise sessions.