Screening and Efficacy Evaluation of Deodorizing Microbes for Manure Waste in Cold Regions: A Case Study of Xinjiang, China.

Conventional deodorizing microbial agents exhibit poor cold tolerance. This study aimed to obtain functional strains capable of degrading ammonia and hydrogen sulfide by screening odor-polluted environments in multiple locations in Xinjiang, China. Deodorizing bacteria were isolated using selective media. Their deodorization efficiency was determined using the zinc ammonium complex salt colorimetric absorption method and the boric acid absorption Kjeldahl nitrogen determination method. The strains were identified through 16S rRNA gene sequence analysis. The obtained strains were then formulated into a deodorizing microbial agent, and their combined deodorization effect was evaluated. Three ammonia-degrading strains were obtained, identified as Serratia proteamaculans, Glutamicibacter bergeri, and Shewanella putrefaciens, with maximum degradation rates reaching 52.62%, 63.81%, and 50.80%, respectively, on day 10. Additionally, two hydrogen sulfide-degrading bacteria belonging to the genus Pseudomonas, designated N16 and N56, were isolated under low-temperature conditions. Strain N16 achieved its highest degradation rate of 43.52% on day 5, while strain N56 reached 57.50% on day 10. A highly efficient deodorizing microbial agent, designated Agent E, was ultimately obtained, exhibiting an ammonia degradation rate of 52.67% and a hydrogen sulfide degradation rate of 78.74%.