Frontiers in insect science · 2026
Ontogenetic variability of the Siberian silk moth, Dendrolimus sibiricus.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Akhanaev Y B, Yakimova M E, Pavlushin S V, Kharlamova D D, Kolosov A V, Ageev A A, Ershov N I, Martemyanov V V
Аннотация
INTRODUCTION: The Siberian silk moth Dendrolimus sibiricus is a major boreal defoliator whose outbreak dynamics vary with the proportion of larvae following 1-year versus 2-year life cycles, yet the physiological basis of this ontogenetic variability remains unclear.
METHODS: We generated tissue-specific RNA sequencing datasets from male and female larvae expressing 1-year or 2-year life cycles, dissected into head, gut, and fat body tissue sets (49 libraries), and quantified life-cycle effects using principal component analysis and differential expression with functional enrichment.
RESULTS: Across tissue sets, transcriptomic profiles were structured primarily by life cycle and secondarily by sex, with the clearest sex-dependent signal confined to gut tissues. The 2-year life-cycle trajectory was characterized by broad down-regulation of genes associated with growth and anabolic metabolism, and up-regulation of stress tolerance, catabolic remodeling, proteasome-related functions, and detoxification modules.
DISCUSSION: In D. sibiricus larvae expressing the 2-year life cycle, transcriptomic patterns indicate that the prolonged larval development underlying this trajectory is associated with a diapause-like state, based on similarities to diapause-associated molecular patterns described in other insects. Laboratory manipulations of larval density, food deprivation, and host plant species did not induce a switch to the 2-year life cycle, suggesting that life-cycle determination depends on more complex cue integration and/or environmental history. Together, these results identify candidate molecular signatures of life-cycle state in D. sibiricus and provide a foundation for future studies of life-cycle regulation in this species.
METHODS: We generated tissue-specific RNA sequencing datasets from male and female larvae expressing 1-year or 2-year life cycles, dissected into head, gut, and fat body tissue sets (49 libraries), and quantified life-cycle effects using principal component analysis and differential expression with functional enrichment.
RESULTS: Across tissue sets, transcriptomic profiles were structured primarily by life cycle and secondarily by sex, with the clearest sex-dependent signal confined to gut tissues. The 2-year life-cycle trajectory was characterized by broad down-regulation of genes associated with growth and anabolic metabolism, and up-regulation of stress tolerance, catabolic remodeling, proteasome-related functions, and detoxification modules.
DISCUSSION: In D. sibiricus larvae expressing the 2-year life cycle, transcriptomic patterns indicate that the prolonged larval development underlying this trajectory is associated with a diapause-like state, based on similarities to diapause-associated molecular patterns described in other insects. Laboratory manipulations of larval density, food deprivation, and host plant species did not induce a switch to the 2-year life cycle, suggesting that life-cycle determination depends on more complex cue integration and/or environmental history. Together, these results identify candidate molecular signatures of life-cycle state in D. sibiricus and provide a foundation for future studies of life-cycle regulation in this species.