Lasers in medical science · 2026
Combination of Bauhinia monandra extract and laser acupuncture reduces carrageenan-induced paw edema.
СтатьяНазвание и аннотация: Перевод в очереди
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da Silva Castro Allyne Aparecida Dias, da Costa Junior Rogério Carvalho, Laurindo Eloisa Acerbi, Soares Lorena Campos, Fernandes Tatyane Matos, Lima Nerilson Marques, Xavier Diego Mendes, Oliveira Murilo Xavier, Castro Sandra Bertelli Ribeiro, de Souza Alves Caio César, de Paula Carli Alessandra
Аннотация
PURPOSE: To investigate the anti-inflammatory effect of the ethanolic extract of Bauhinia monandra Kurz (EEB) alone and combined with laser acupuncture (LA) in a carrageenan-induced paw edema model.
METHODS: EEB was characterized using HPLC-HRMS and tested for cell viability and nitric oxide (NO) production in RAW 264.7 and J774A.1 macrophages. In vivo, female BALB/c mice with paw edema were treated with EEB (100 or 200 mg/kg) alone, combined with LA (808 nm, 120 mW, 3 J, 25 s) at acupoints ST36 and SP6, or local laser. Paw edema was measured up to 4 h post-induction, and cytokine levels (IL-1$\beta$ and IL-6) were quantified.
RESULTS: EEB showed no cytotoxicity and significantly reduced NO production in vitro. Phytochemical analysis revealed a predominance of phenolic compounds, including flavonoids. In vivo, both EEB and LA significantly reduced edema. Notably, the EEB200 + LA group produced reductions in paw edema and pro-inflammatory cytokines comparable to the dexamethasone group.
CONCLUSION: EEB demonstrated anti-inflammatory activity both in vitro and in vivo. Combining EEB (200 mg/kg) with LA yielded a more consistent anti-inflammatory response than either treatment alone, particularly in reducing edema and pro-inflammatory cytokines. These findings support further investigation, though larger sample sizes and chronic models are required before clinical claims.
METHODS: EEB was characterized using HPLC-HRMS and tested for cell viability and nitric oxide (NO) production in RAW 264.7 and J774A.1 macrophages. In vivo, female BALB/c mice with paw edema were treated with EEB (100 or 200 mg/kg) alone, combined with LA (808 nm, 120 mW, 3 J, 25 s) at acupoints ST36 and SP6, or local laser. Paw edema was measured up to 4 h post-induction, and cytokine levels (IL-1$\beta$ and IL-6) were quantified.
RESULTS: EEB showed no cytotoxicity and significantly reduced NO production in vitro. Phytochemical analysis revealed a predominance of phenolic compounds, including flavonoids. In vivo, both EEB and LA significantly reduced edema. Notably, the EEB200 + LA group produced reductions in paw edema and pro-inflammatory cytokines comparable to the dexamethasone group.
CONCLUSION: EEB demonstrated anti-inflammatory activity both in vitro and in vivo. Combining EEB (200 mg/kg) with LA yielded a more consistent anti-inflammatory response than either treatment alone, particularly in reducing edema and pro-inflammatory cytokines. These findings support further investigation, though larger sample sizes and chronic models are required before clinical claims.