Biphasic Interfacial Transmission Enables DNA Storage in Liquid.

DNA is recognized to be a high-density storage medium for solving the global data storage crisis. From encoding to storage, massive information stored in synthetic DNA requires low-loss and high-speed transmission to a reliable preservation environment. Minimizing energy consumption remains a key challenge throughout this process. In this study, an aqueous biphasic interfacial transmission (BIT) pathway is established that enables simultaneous salt-decontaminated migration and anti-aging preservation, with a theoretically extrapolated half-life of up to 577 years at -20°C. Specific temperature windows of either 20°C, 25°C, or 30-40°C open the trans-phasic pathway. Covering most room-temperature scenarios without excess energy input, the process is completed at a 30-minute standing solution state or accelerated by a 2-minute centrifugation mode. The interphase partition coefficient exceeds 264, ensuring a DNA transfer efficiency of over 80% regardless of DNA structure or sequence. The low water activity of the PEG-rich preservation environment stabilizes the nucleotide bonding, thus preserving the integrity of DNA's secondary structure information throughout the entire phase transferring process. The polymeric preservation network kinetically restricts DNA and enzyme diffusion, isolating degradation factors. This BIT pathway enables a scalable and energy-efficient strategy with tunable operational parameters for securing DNA data storage during information circulation and long-term preservation.