One-dimensional single-photon position-sensitive silicon photomultiplier and its application in Raman spectroscopy.

Curcumin is the active compound of one of the most widely used spices (turmeric) in the world and it is thought to also possess anti-fungal and other biological activities. However, the molecular and cellular targets of curcumin remain unknown. Here, we undertook a novel imaging-based approach to determine the intracellular distribution of curcumin using the model organism fission yeast Schizosaccharomyces pombe by taking advantage of the intrinsic fluorescence of curcumin. Live fluorescence microscopy revealed for the first time that curcumin, at a concentration of just 1 μM, formed a narrow circumferential ring around the equatorial plane of dividing cells within minutes after being added to the yeast culture. The intensity of this ring increased proportionally to the concentration of curcumin and gradually over time. The curcumin ring colocalized both spatially and temporally with septin ring and the exocyst complex at the equatorial plane during cytokinesis. Deletion of one of the septin genes (spn4) reduced the frequency of curcumin ring formation by 74%. Micromolar concentrations of curcumin slowed down the contractile ring constriction by up to 49% in a dosage-dependent manner. Besides fission yeast, curcumin similarly targets the division plane of two other yeasts, Saccharomyces cerevisiae and Candida albicans. Thus, curcumin, originating from plants, targets the septin cytoskeleton to delay yeast cytokinesis, suggesting its potential anti-fungal activity and use as a fluorescence probe for yeast septins.