Assessing Urinary Microbiota in Chronic Cystitis Based on Midstream Urine Sample.

INTRODUCTION

This study aimed to compare the urinary microbiota of healthy women, women with a predisposition to UTIs, and patients with chronic recurrent cystitis using real-time PCR, as well as identify diagnostic markers for urinary diseases.

METHODS

The study enrolled 3 groups of patients: a healthy control group, patients with chronic recurrent cystitis, and patients with a risk of developing UTIs. Urine samples were analyzed by multiplex real-time PCR reagent kits Femoflor®16 and BacScreen OM.

RESULTS

Chronic recurrent cystitis is associated with an increase in total bacterial mass (TBM), genomic DNA, and relative predominance of facultative anaerobic microorganisms. The most prevalent bacterial species found in chronic cystitis was Escherichia coli in conjunction with other Enterobacteriaceae, most commonly Serratia marcescens. An increased amount of genomic DNA and both facultative and obligate anaerobic microorganisms was observed in patients with a risk of developing UTIs. A relative decrease in Lactobacillus spp. was noted in both groups, with the chronic cystitis group showing a more pronounced reduction.

CONCLUSION

In summary, the levels of genomic DNA, TBM, and relative values of Lactobacillus spp. can be used as molecular diagnostic markers for chronic cystitis and a variety of other conditions, including micronephrolithiasis and bacterial vaginosis.