Dual-mechanism driven adsorption on silica microspheres enables precision pharmacokinetic profiling of liposomal therapeutics.

• SiO2 microspheres accurately separated encapsulated, free, and total liposomal drug forms in plasma with cost efficiency. • Silica hydroxyl and siloxane groups bind liposomal phospholipids via dual hydrogen bonding and electrostatic interactions. • The method offers high sensitivity, precision, and recovery for pharmacokinetic analysis of the three liposomal drug forms. • This validated approach may be developed into a universal method for liposomal drugs.