[Establishment of an integrin β3-overexpressing cell line and its influence on Hantaan virus infection and replication].

Objective To establish a stable cell line overexpressing integrin β3(ITGB3), aiming to overcome the limited replication efficiency and low viral yields of Hantaan virus (HTNV) in conventional cell culture systems. Methods A lentiviral vector encoding ITGB3 was constructed and packaged into viral particles. Vero E6 and A549 cells were transduced and selected with puromycin to generate stable lines. ITGB3 expression was verified by quantitative RT-qPCR, Western blot, and immunofluorescence staining, and cell viability was evaluated using the CCK-8 assay. Following HTNV infection, viral replication was assessed by measuring viral RNA copies, nucleoprotein (NP) expression, and virus titers. Results ITGB3 was stably and highly expressed in Vero E6 and A549 cells, with no significant impact on cell proliferation. After HTNV infection, the levels of viral RNA replication and the expression of NP protein were both significantly upregulated in the ITGB3-overexpressing group, and the virus titers were markedly higher than those in the control group. Conclusion The established ITGB3 overexpressing cell line promotes HTNV replication and propagation, providing a reliable and efficient model for investigating HTNV pathogenesis and facilitating the development of antiviral drugs.