Indian journal of public health · 2025
Shiga Toxin Profiles of Poultry-origin Escherichia coli Isolates with Low Levels of Antimicrobial Resistance to the Clinical and Laboratory Standard Institute-recommended Antibiotics for Surveillance in Human Beings.
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Soodan Maansi, Rana Kavita, Panda Ashok Kumar, Dinesh Krishanender, Kumar Rajesh, Vaid Rajesh Kumar, Sankhyan Varun, Thakur Sidharath Dev
Аннотация
BACKGROUND: Escherichiacoli are the reservoirs of antimicrobial resistance (AMR) genes. Shiga toxin-producing E. coli of animal origin can cause severe life-threatening infections in man.
OBJECTIVES: The present study investigated the susceptibility of poultry-origin E. coli isolates to Clinical and Laboratory Standard Institute (CLSI)-recommended antibiotics and their Shiga toxin profiles.
MATERIALS AND METHODS: A total of 141 samples comprising cloacal swabs and water and litter samples were investigated. Antimicrobial susceptibility profiles of the recovered isolates were determined for 15 antibiotics as recommended by CLSI. Shiga toxin-encoding genes (Shiga toxin 1 [ stx1 ] and Shiga toxin 2 [ stx2 ]) and plasmid-mediated AMR determinants were ascertained by the polymerase chain reaction.
RESULTS: E. coli isolation rates were 90.1% with a higher incidence in organized (93.8%) compared to backyard rearing systems (87%). AMR (21.3%) was limited to only three antibiotic classes, i.e., penicillins (9.4%), fluoroquinolones (11.0%), and phenicols (0.8%). All the isolates were susceptible to aminoglycosides, carbapenems, monobactams, and polymyxins with the absence of multidrug resistance. The incidence of resistant E. coli was significantly higher in organized rearing systems (30.0%) compared to backyard units (13.4%). All penicillin-resistant isolates were positive for bla SHV and two isolates co-expressed bla OXA-1 with bla SHV . 21.4% of fluoroquinolone-resistant isolates were positive for plasmid-mediated aac(6')-Ib resistance. 96.3% of resistant isolates carried isolates stx2 and none had stx1 .
CONCLUSION: Low AMR incidence was recorded in E. coli isolates of poultry origin for CLSI-recommended old and newer classes of antibiotics. Higher levels of stx2 detections in antimicrobial-resistant E. coli isolates pose a significant public health threat.
OBJECTIVES: The present study investigated the susceptibility of poultry-origin E. coli isolates to Clinical and Laboratory Standard Institute (CLSI)-recommended antibiotics and their Shiga toxin profiles.
MATERIALS AND METHODS: A total of 141 samples comprising cloacal swabs and water and litter samples were investigated. Antimicrobial susceptibility profiles of the recovered isolates were determined for 15 antibiotics as recommended by CLSI. Shiga toxin-encoding genes (Shiga toxin 1 [ stx1 ] and Shiga toxin 2 [ stx2 ]) and plasmid-mediated AMR determinants were ascertained by the polymerase chain reaction.
RESULTS: E. coli isolation rates were 90.1% with a higher incidence in organized (93.8%) compared to backyard rearing systems (87%). AMR (21.3%) was limited to only three antibiotic classes, i.e., penicillins (9.4%), fluoroquinolones (11.0%), and phenicols (0.8%). All the isolates were susceptible to aminoglycosides, carbapenems, monobactams, and polymyxins with the absence of multidrug resistance. The incidence of resistant E. coli was significantly higher in organized rearing systems (30.0%) compared to backyard units (13.4%). All penicillin-resistant isolates were positive for bla SHV and two isolates co-expressed bla OXA-1 with bla SHV . 21.4% of fluoroquinolone-resistant isolates were positive for plasmid-mediated aac(6')-Ib resistance. 96.3% of resistant isolates carried isolates stx2 and none had stx1 .
CONCLUSION: Low AMR incidence was recorded in E. coli isolates of poultry origin for CLSI-recommended old and newer classes of antibiotics. Higher levels of stx2 detections in antimicrobial-resistant E. coli isolates pose a significant public health threat.