[Acid hydrolase activity and permeability of lysosomal membranes of cardiomyocytes and hepatocytes in extreme states].

Activity of some lysosomal enzymes was studied in the primary heart cell culture of newborn rats under conditions of hypoxia and in isolated ischemic perfused heart, in liver and heart tissues of dogs after traumatic shock and after experimental myocardium infarction as well as in liver tissue of rats after 10 min experimental heart arrest and during the postresuscitation period. Decrease in cAMP and elevation in cGMP levels, impairment of the adenylate cyclase hormonal regulation, decrease in activity of cAMP-dependent protein kinases were shown to correlate distinctly with an increase of acid hydrolases free activity in liver and heart cell cytoplasm of dogs with traumatic shock and with experimental myocardium infarction. Free activity of acid phosphatase was increased in primary heart cell culture under conditions of hypoxia and in isolated ischemic perfused rat heart. Intraperitoneal administration of cAMP-containing liposomes into the rats with 10 min heart arrest led to stabilization of liver lysosomal membranes within 1 hr of 4 hrs of the post-resuscitation period. The mechanisms involved in stabilization of lysosomal membranes are discussed.