Construction of hybrid chiral microcompartment of Au NPs grafted penicillamine and BSA-PNIPAAm conjugate with enantiomatically modulated interfacial catalytic efficiency.

Cell chirality is a complex phenomenon that imparts unique properties. The creation of biomimetic artificial cells chirality is a rapidly evolving research topic that has had a profound impact in multiple fields, including materials science, catalysis, biotechnology, and biomedicine. Herein, we report a simple method that combines L- or D-penicillamine (L/D-Pen) with proteinosomes on the surface of gold nanoparticles to afford nanoparticle hybrid microcapsules. Encapsulation of the enzyme in the microcapsule enables a chiral recognition effect on the membrane surface, thereby affording control over the enantioselective catalytic reaction of the d-glucose (D-Glu) substrate loaded inside the hybrid microcapsule. The adsorption energy of D-Glu and D-Pen (-0.095) calculated by MS is less than that of D-Glu and L-Pen (-0.064). Thus enantiomeric selective recognition is a stronger interaction force than adsorption for the same configuration. These findings highlight the development of biomimetic chiral microcompartments, and provide a platform for driving cellular chirality in various organisms.