Didymin mitigates neuroinflammation and preserves blood-brain barrier integrity after subarachnoid hemorrhage.

OBJECTIVE

Subarachnoid hemorrhage (SAH) is a highly lethal and disabling type of stroke. The main causes of poor prognosis are neuroinflammation, blood-brain barrier (BBB) disruption and brain edema following hemorrhage. Didymin has shown neuroprotective effects in intracerebral hemorrhage; however, its regulatory role in SAH remains unclear.

METHODS

The rat SAH model was established using the internal carotid artery puncture method, while an in vitro model was developed by stimulating human brain microvascular endothelial cells (HBMECs) with hemoglobin (Hb). Following didymin treatment, neurological functional outcomes were assessed using the modified Garcia score and the balance beam test. Nissl staining was performed to evaluate neuronal pathological changes. Immunofluorescence staining was employed to assess microglial activation and BBB integrity. Brain water content was measured to evaluate the severity of cerebral edema. Western blot analysis was utilized to detect the expression of matrix metalloproteinase 9 (MMP9), apoptosis-related proteins (Bcl-XL, Bcl-2, Bax), pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), and tight junction proteins (ZO-1, Occludin).

RESULTS

Didymin treatment significantly improved neurological function scores in SAH rats by alleviating neuronal damage and apoptosis. On one hand, didymin reduced post-SAH neuroinflammation by inhibiting excessive microglial activation and the expression of pro-inflammatory cytokines. On the other hand, didymin preserved BBB integrity and alleviated brain edema by downregulating MMP9 expression. In Hb-induced cell model, didymin suppressed MMP9 expression and promoted the expression of tight junction proteins.

CONCLUSION

In this study, we demonstrated that didymin mitigates neuronal damage and apoptosis following SAH, effectively suppresses neuroinflammation, maintains the integrity of the BBB, and attenuates brain edema. These findings suggest that didymin holds promise as a potential therapeutic candidate for the treatment of SAH.