Monozygotic multiple gestation after intracytoplasmic sperm injection and preimplantation genetic diagnosis.

OBJECTIVE

To report a possible association between intracytoplasmic sperm injection (ICSI)-preimplantation genetic diagnosis (PGD) and monozygotic multiple gestation.

DESIGN

Small case series.

SETTING

In vitro fertilization unit in an academic medical center.

PATIENT(S): Three patients were treated with ICSI-PGD for sexing as well as selection against a known translocation.

INTERVENTION(S): Transfer of day 4 embryos to the uterus.

MAIN OUTCOME MEASURE(S): Clinical pregnancy.

RESULT(S): Two pairs of monozygotic twins and a triplet pregnancy.

CONCLUSION(S): Repeated manipulation of the zona pellucida as well as extended embryo culture during ICSI-PGD treatments may result in monozygotic twin and triplet pregnancies.