An allosteric DNAzyme-Based logic circuit intelligent electrochemiluminescence biosensor for the precise diagnosis of acute myocardial infarction.

Acute myocardial infarction (AMI) is a life-threatening medical emergency that requires early diagnosis and prompt intervention. However, distinguishing AMI from other coronary artery diseases with similar clinical manifestations remains challenging. In this study, a novel electrochemiluminescence (ECL) biosensor was developed by integrating allosteric DNAzyme nanodevices with ECL resonance energy transfer (ECL-RET) probes to enable intelligent analysis of multiple miRNAs related to different coronary artery diseases. A series of DNA-based logic devices was constructed by integrating strand displacement reactions into a DNAzyme framework, including YES, AND, OR, and INHIBIT logic gates. The "INHIBIT-AND" logic circuits, which perform complex information processing, can effectively analyze multiple miRNAs and intelligently differentiate diseases similar to AMI. The final signal was detected using an ECL-RET probe synthesized by encapsulating Ru[(bpy)3]2+ reductive-oxidative (R-O) emitters and CdTe quantum dots (QD) oxidative-reductive (O-R) emitters into DNA-modified silica nanoparticles. The sensing platform can intelligently detect multiple miRNAs, with a detection limit of 62 aM (S/N = 3), ranging from 1 fM to 1 nM. This strategy was applied to patients' serum samples, providing a new idea for the autonomous and precise diagnosis of AMI.