Neonatology · 2007
Effect of Acute Administration of Recombinant Human Leptin during the Neonatal Period on Body Temperature and Endocrine Profile of the Piglet.
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Litten J C, Mostyn A, Laws J, Corson A M, Symonds M E, Clarke L
Аннотация
BACKGROUND: Leptin is produced predominantly by white adipocytes; in adults it regulates appetite and energy expenditure but its role in the neonate remains to be fully established.
OBJECTIVES: To examine the effects of acute administration of recombinant human leptin on the endocrine profile and thermoregulation of neonatal pigs.
METHODS: 24 pairs of siblings (n = 48) were administered with either a single dose (4 microg ml(-1) kg(-1) body weight) of leptin (L: n = 24) or a placebo (P: n = 24) on day 6 of neonatal life. Rectal temperature was recorded, and tissue samples were taken at 1 (n = 12), 2 (n = 12), 4 (n = 12) or 6 (n = 12) hours post-administration. Plasma concentrations of hormones and metabolites were determined in conjunction with messenger RNA (mRNA) for leptin and uncoupling protein-2.
RESULTS: Plasma leptin increased following leptin administration, and differences in concentrations of insulin, thyroxine and non-esterified fatty acids were observed between the two groups. Initially, rectal temperature decreased in L pigs but returned to start values by 1.5 h. This decline in rectal temperature was delayed in placebo animals, resulting in differences between treatments at 1.5 and 2 h.
CONCLUSIONS: Acute leptin administration alters the endocrine profile of pigs and influences the thermoregulatory ability of the neonate.
OBJECTIVES: To examine the effects of acute administration of recombinant human leptin on the endocrine profile and thermoregulation of neonatal pigs.
METHODS: 24 pairs of siblings (n = 48) were administered with either a single dose (4 microg ml(-1) kg(-1) body weight) of leptin (L: n = 24) or a placebo (P: n = 24) on day 6 of neonatal life. Rectal temperature was recorded, and tissue samples were taken at 1 (n = 12), 2 (n = 12), 4 (n = 12) or 6 (n = 12) hours post-administration. Plasma concentrations of hormones and metabolites were determined in conjunction with messenger RNA (mRNA) for leptin and uncoupling protein-2.
RESULTS: Plasma leptin increased following leptin administration, and differences in concentrations of insulin, thyroxine and non-esterified fatty acids were observed between the two groups. Initially, rectal temperature decreased in L pigs but returned to start values by 1.5 h. This decline in rectal temperature was delayed in placebo animals, resulting in differences between treatments at 1.5 and 2 h.
CONCLUSIONS: Acute leptin administration alters the endocrine profile of pigs and influences the thermoregulatory ability of the neonate.
Предметные рубрики MeSH
3-Hydroxybutyric AcidAnimalsAnimals, NewbornBlood GlucoseBody CompositionBody Temperature RegulationBody WeightEndocrine SystemFatty Acids, NonesterifiedFemaleInsulinIon ChannelsLactatesLeptinMaleMitochondrial ProteinsRNARandom AllocationRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionSwineThyroxineTriglyceridesTriiodothyronineUncoupling Protein 2