Therapeutic potential of goat whey protein hydrolysate and vitamin-D3 in combating D-galactose-induced sperm senescence in male mice.

OBJECTIVE

To evaluate the therapeutic synergistic effect of goat whey protein hydrolysate (WPH) and vitamin-D3 (Vit-D3) in mitigating the adverse effects of D-galactose-induced sperm senescence in male mice (effects on sperm quality and reproductive health of male mice).

DESIGN

Controlled experiment animal study conducted on male Swiss albino mice to assess the synergistic effects of WPH and Vit-D3 against D-galactose-induced sperm senescence.

SUBJECTS: The present in vivo study was designed to evaluate the synergistic effect of WP, WPH, and Vit-D3 on sperm function and motility in male mice. In this study, 48 male Swiss albino mice were randomly assigned to eight groups.

EXPOSURE: To induce aging in mice, a solution of 0.9% saline containing D-galactose (150 mg/kg body weight) was injected intraperitoneally for 8 weeks. In this experiment, 48 male Swiss albino mice were randomly categorized into eight groups (I: control; II: D-Galactose (150 mg/kg body weight), Group III: Vit-C (300 mg/kg body weight), Group IV: WP (800 mg/kg body weight), Group V: WPH (800 mg/kg body weight), Group VI; Vit-D3 (2 ng/μL oral), Group VII; WP+ Vit-D3, and Group VIII; WPH+Vit-D3. MAIN OUTCOME MEASURES: Evaluation of sperm quality parameters: sperm survival, sperm progressive motility, hypo-osmotic swelling test analysis, and testosterone level.

RESULTS

Results indicate a significant increase in the ratio of sperm survival, sperm progressive motility, and testosterone level by supplementation of WP, WPH, and Vit-D3. These exposures, including dietary supplementation, have demonstrated promising results in mitigating the effects of aging on the reproductive health of mice.

CONCLUSION

This study contributes to enhanced male reproductive health by diminishing the impact of senescence-induced male infertility and could be considered a potential therapeutic approach for healthy male reproduction.