A characteristic bacterial SERS marker for direct identification of Salmonella in real samples assisted by a high-performance SERS chip and a selective culture medium.

Salmonella should be absent in pharmaceutical preparations and foods according to the regulations. However, up to now, rapid and convenient identification of Salmonella is still full of challenge. Herein, we reported a label-free surface-enhanced Raman scattering (SERS) method for direct identification of Salmonella spiked in drug samples based on a characteristic bacterial SERS marker assisted by a high-performance SERS chip and a selective culture medium. The SERS chip being fabricated through in situ growth of bimetallic Au-Ag nanocomposites on silicon wafer within 2 h, featured a high SERS activity (EF > 107), good uniformity and batch-to-batch consistency (RSD < 10 %), and satisfactory chemical stability. The directly-visualized SERS marker at 1222 cm-1 originated from bacterial metabolite hypoxanthine was robust and exclusive for discrimination of Salmonella with other bacterial species. Moreover, the method was successfully used for direct discrimination of Salmonella in mixed pathogens by using a selective culture medium, and could identify Salmonella contaminant at ∼1 CFU spiked level in a real sample (Wenxin granule, a botanical drug) after 12 h of enrichment. The combined results showed that developed SERS method is practical and reliable, and could be a promising alternative for rapid identification of Salmonella contamination in pharmaceutical and foods industries.