Journal of advanced research · 2026
A targeted siderophore Sperbactin from Lysobacter enzymogenes OH11: Characterization and biocontrol application against rice bacterial diseases.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Tang Bao, Laborda Pedro, Chen Xian, Gao Jian, Liu Fengquan
Аннотация
INTRODUCTION: Lysobacter enzymogenes OH11 is widely recognized for its potent antagonistic activity against diverse plant pathogens, including bacteria, fungi, and oomycetes. However, the specific compound responsible for its activity against Gram-negative bacteria had remained unidentified.
OBJECTIVES: This study aimed to identify the specific anti-Gram-negative bacterial compound produced by OH11 and to evaluate its potential as a biocontrol agent.
METHODS: Methods included MS/NMR for structure elucidation, antagonism/greenhouse/UV-vis assays for function evaluation, medium/precursor optimization for yield enhancement, and gene knockout/enzyme assays for biosynthetic pathway identification.
RESULTS: The isolated compound was identified as a spermidine-containing siderophore, designated Sperbactin. Through broad-spectrum screening, Sperbactin was discovered to exert highly targeted antagonistic effects specifically against rice pathogens (Xanthomonas oryzae). In greenhouse trials, Sperbactin demonstrated remarkable efficacy against rice bacterial blight, achieving a disease control efficacy of 85.31%, which is comparable to conventional chemical agents. The Sperbactin-Fe(III) complex was found to have a 1:1 M ratio and displayed a high affinity for ferric iron, with an association constant (Ka) of 2.16 × 1029 M-1. Furthermore, the fermentation yield of Sperbactin was increased by 3.29-fold, reaching 290.46 ± 6.22 mg/L. spbB and spbD were identified as essential genes for Sperbactin biosynthesis.
CONCLUSION: This study identifies Sperbactin as a highly promising, narrow-spectrum biocontrol candidate for the targeted management of rice bacterial diseases. Its development offers a sustainable and eco-friendly alternative to chemical pesticides, supporting the advancement of sustainable agriculture.
OBJECTIVES: This study aimed to identify the specific anti-Gram-negative bacterial compound produced by OH11 and to evaluate its potential as a biocontrol agent.
METHODS: Methods included MS/NMR for structure elucidation, antagonism/greenhouse/UV-vis assays for function evaluation, medium/precursor optimization for yield enhancement, and gene knockout/enzyme assays for biosynthetic pathway identification.
RESULTS: The isolated compound was identified as a spermidine-containing siderophore, designated Sperbactin. Through broad-spectrum screening, Sperbactin was discovered to exert highly targeted antagonistic effects specifically against rice pathogens (Xanthomonas oryzae). In greenhouse trials, Sperbactin demonstrated remarkable efficacy against rice bacterial blight, achieving a disease control efficacy of 85.31%, which is comparable to conventional chemical agents. The Sperbactin-Fe(III) complex was found to have a 1:1 M ratio and displayed a high affinity for ferric iron, with an association constant (Ka) of 2.16 × 1029 M-1. Furthermore, the fermentation yield of Sperbactin was increased by 3.29-fold, reaching 290.46 ± 6.22 mg/L. spbB and spbD were identified as essential genes for Sperbactin biosynthesis.
CONCLUSION: This study identifies Sperbactin as a highly promising, narrow-spectrum biocontrol candidate for the targeted management of rice bacterial diseases. Its development offers a sustainable and eco-friendly alternative to chemical pesticides, supporting the advancement of sustainable agriculture.