bioRxiv : the preprint server for biology · 2025
Custom-Built Electrodes Perform Comparably to a Discontinued Commercial Electrode for Neuromuscular Electrical Stimulation in Mice.
СтатьяПрепринтНазвание и аннотация: Перевод в очереди
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Odeh Bana H, Wellman Amanda L, Ameye Michael, Atwood Zachary, Gray Luke, Saravanan Aiswarya, Poluru Havish, Begam Morium, Jones Takako I, Roche Renuka, Roche Joseph A
Аннотация
OBJECTIVE: A commercial electrode used for transcutaneous neuromuscular electrical stimulation (NMES) in mice is no longer available. In response, we developed two low-cost, customizable alternatives-a 3D-Printed Electrode and a Pen Electrode assembled from off-the-shelf jumper wire components-and evaluated their performance relative to the discontinued commercial standard.
METHODS: We conducted in vivo NMES of the left hindlimb ankle dorsiflexors in C57BL/6J mice using three electrode types: a Simple Electrode (previously available as BS4 50-6824, Harvard Apparatus), our novel 3D-Printed Electrode, and our novel Pen Electrode. Contractile torque was recorded during twitch and tetanic contractions, and electrode performance was evaluated based on peak torque values. A repeated measures ANOVA was used to compare torque generated by each animal for the three different types of electrodes.
RESULTS: Both custom electrodes generated twitch and tetanic responses comparable to those of the Commercial electrode. No statistically significant differences were observed in peak torque values in either twitch (p = 0.181) or tetany (p = 0.438).
CONCLUSION: Custom-built 3D-printed and Pen-style electrodes offer low-cost, accessible alternatives to discontinued commercial devices for murine NMES studies. These tools can facilitate continued use of electrical stimulation protocols in preclinical neuromuscular research.
METHODS: We conducted in vivo NMES of the left hindlimb ankle dorsiflexors in C57BL/6J mice using three electrode types: a Simple Electrode (previously available as BS4 50-6824, Harvard Apparatus), our novel 3D-Printed Electrode, and our novel Pen Electrode. Contractile torque was recorded during twitch and tetanic contractions, and electrode performance was evaluated based on peak torque values. A repeated measures ANOVA was used to compare torque generated by each animal for the three different types of electrodes.
RESULTS: Both custom electrodes generated twitch and tetanic responses comparable to those of the Commercial electrode. No statistically significant differences were observed in peak torque values in either twitch (p = 0.181) or tetany (p = 0.438).
CONCLUSION: Custom-built 3D-printed and Pen-style electrodes offer low-cost, accessible alternatives to discontinued commercial devices for murine NMES studies. These tools can facilitate continued use of electrical stimulation protocols in preclinical neuromuscular research.