Folia morphologica · 2024
An investigation of the effectiveness of atmospheric pressure cold plasma on sciatic nerve injury in rats.
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Yılmaz Nesibe, Deniz Omur Gulsum, Secgin Seyda, Secgin Yusuf, Bozduman Ferhat
Аннотация
BACKGROUND: The aim of this study was to evaluate the efficacy of atmospheric pressure cold plasma jet and plasma activated medium (PAM) on sciatic nerve injury (SNI).
MATERIALS AND METHODS: Rats were divided into 6 groups (n = 10); group 1 (sham), group 2 (SNI), group 3 (SNI + atmospheric pressure cold plasma jet 5 min), group 4 (SNI + atmospheric pressure cold plasma jet 10 min), group 5 (SNI + PAM 5 min), and group 6 (SNI + PAM 10 min). On days 1, 8, 15, and 22 of the study, atmospheric pressure cold plasma jet was applied to rats in groups 3 and 4, and PAM was applied to rats in groups 5 and 6. A hot plate test was applied to all rats on the same days. On day 28, the experiment was terminated, and sciatic nerve tissues were removed for histopathological evaluation.
RESULTS: According to the 4-week average of the hot plate tests, a significant relationship was found between group 2 and group 4 and group 6 (p < 0.05). When evaluated within each week, significant differences were found between group 2 and group 4 in week 1, between group 2 and group 5 and group 6 in week 2, between group 2 and group 4 in week 3, and between group 2 and group 4 and group 6 in week 4 (p < 0.05). As a result of histopathological analysis, except for the control group, the other groups had similar characteristics in terms of axonal degeneration, periaxonal swelling, and axon density.
CONCLUSIONS: As a result of our study, we found that plasma application showed an improvement in the duration of the hot plate test but did not show any improvement histopathologically.
MATERIALS AND METHODS: Rats were divided into 6 groups (n = 10); group 1 (sham), group 2 (SNI), group 3 (SNI + atmospheric pressure cold plasma jet 5 min), group 4 (SNI + atmospheric pressure cold plasma jet 10 min), group 5 (SNI + PAM 5 min), and group 6 (SNI + PAM 10 min). On days 1, 8, 15, and 22 of the study, atmospheric pressure cold plasma jet was applied to rats in groups 3 and 4, and PAM was applied to rats in groups 5 and 6. A hot plate test was applied to all rats on the same days. On day 28, the experiment was terminated, and sciatic nerve tissues were removed for histopathological evaluation.
RESULTS: According to the 4-week average of the hot plate tests, a significant relationship was found between group 2 and group 4 and group 6 (p < 0.05). When evaluated within each week, significant differences were found between group 2 and group 4 in week 1, between group 2 and group 5 and group 6 in week 2, between group 2 and group 4 in week 3, and between group 2 and group 4 and group 6 in week 4 (p < 0.05). As a result of histopathological analysis, except for the control group, the other groups had similar characteristics in terms of axonal degeneration, periaxonal swelling, and axon density.
CONCLUSIONS: As a result of our study, we found that plasma application showed an improvement in the duration of the hot plate test but did not show any improvement histopathologically.