Journal of veterinary internal medicine · 2026
Veterinary blood culture diagnostic testing: methodology and results from a German single-center study on dogs (2014-2022).
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Sandbrink Teresa, Lübke-Becker Antina, Robé Caroline, Fulde Marcus, Knopf Myriam Antonia, Merle Roswitha, Weingart Christiane, Rösler Uwe, Schwarz Stefan, Kohn Barbara
Аннотация
BACKGROUND: Blood cultures (BCs) are the gold standard for identifying bacterial bloodstream infections and are vital for directing antimicrobial treatment.
HYPOTHESIS/OBJECTIVES: Characterize BC results and diagnostic testing in veterinary medicine. Pathogen identification (ID) results, antimicrobial susceptibility testing (AST) results, and management factors were investigated to identify optimization opportunities.
ANIMALS: A total of 750 BCs from 687 dogs presented between January 2014 and September 2022 at a German teaching hospital, with 102 positive BCs from 101 dogs.
METHODS: Retrospective analysis of historical data. A BC system suitable for aerobic and anaerobic pathogens was used.
RESULTS: Blood culture yield (clinically relevant growth in 102/750 BCs) was 13.6%, and the contamination rate was 1.9%. Commonly isolated pathogens were Enterobacterales (n = 15/25 multidrug-resistant), coagulase-positive staphylococci (n = 3/23 methicillin-resistant), beta-hemolytic streptococci (n = 19), and obligate anaerobes (n = 22). Polymicrobial growth occurred in 8 BCs (7.8%). Median duration of the preanalytical phase (sampling until receipt in laboratory) was 1 day (interquartile range [IQR], 1-2), from receipt in laboratory until ID results 1 day (IQR, 1-2), and until AST results 2 days (IQR, 2-4). The preanalytical phase of BCs taken on weekends was a median of 1 day longer (P < .001). Dogs treated with antimicrobials within 7 days before sampling were 10.74 times more likely to yield a multidrug-resistant isolate (95% confidence interval [CI], 3.62-31.86).
CONCLUSIONS AND CLINICAL IMPORTANCE: Improving BC processing requires addressing the management factors BC yield, contamination rate, weekend sampling, and antimicrobial pretreatment. Detection of resistant bacteria emphasizes the need for more rapid processing to shorten the time to optimized antimicrobial treatment.
HYPOTHESIS/OBJECTIVES: Characterize BC results and diagnostic testing in veterinary medicine. Pathogen identification (ID) results, antimicrobial susceptibility testing (AST) results, and management factors were investigated to identify optimization opportunities.
ANIMALS: A total of 750 BCs from 687 dogs presented between January 2014 and September 2022 at a German teaching hospital, with 102 positive BCs from 101 dogs.
METHODS: Retrospective analysis of historical data. A BC system suitable for aerobic and anaerobic pathogens was used.
RESULTS: Blood culture yield (clinically relevant growth in 102/750 BCs) was 13.6%, and the contamination rate was 1.9%. Commonly isolated pathogens were Enterobacterales (n = 15/25 multidrug-resistant), coagulase-positive staphylococci (n = 3/23 methicillin-resistant), beta-hemolytic streptococci (n = 19), and obligate anaerobes (n = 22). Polymicrobial growth occurred in 8 BCs (7.8%). Median duration of the preanalytical phase (sampling until receipt in laboratory) was 1 day (interquartile range [IQR], 1-2), from receipt in laboratory until ID results 1 day (IQR, 1-2), and until AST results 2 days (IQR, 2-4). The preanalytical phase of BCs taken on weekends was a median of 1 day longer (P < .001). Dogs treated with antimicrobials within 7 days before sampling were 10.74 times more likely to yield a multidrug-resistant isolate (95% confidence interval [CI], 3.62-31.86).
CONCLUSIONS AND CLINICAL IMPORTANCE: Improving BC processing requires addressing the management factors BC yield, contamination rate, weekend sampling, and antimicrobial pretreatment. Detection of resistant bacteria emphasizes the need for more rapid processing to shorten the time to optimized antimicrobial treatment.