Cell stem cell · 2024
Fully biologic endothelialized-tissue-engineered vascular conduits provide antithrombotic function and graft patency.
СтатьяПоддержка правительства СШАПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Park Jinkyu, Riaz Muhammad, Qin Lingfeng, Zhang Wei, Batty Luke, Fooladi Saba, Kural Mehmet H, Li Xin, Luo Hangqi, Xu Zhen, Wang Juan, Banno Kimihiko, Gu Sean X, Yuan Yifan, Anderson Christopher W, Ellis Matthew W, Zhou Jiahui, Luo Jiesi, Shi Xiangyu, Shin Jae Hun, Liu Yufeng, Lee Seoyeon, Yoder Mervin C, Elder Robert W, Mak Michael, Thorn Stephanie, Sinusas Albert, Gruber Peter J, Hwa John, Tellides George, Niklason Laura E, Qyang Yibing
Аннотация
Tissue-engineered vascular conduits (TEVCs), often made by seeding autologous bone marrow cells onto biodegradable polymeric scaffolds, hold promise toward treating single-ventricle congenital heart defects (SVCHDs). However, the clinical adoption of TEVCs has been hindered by a high incidence of graft stenosis in prior TEVC clinical trials. Herein, we developed endothelialized TEVCs by coating the luminal surface of decellularized human umbilical arteries with human induced pluripotent stem cell (hiPSC)-derived endothelial cells (ECs), followed by shear stress training, in flow bioreactors. These TEVCs provided immediate antithrombotic function and expedited host EC recruitment after implantation as interposition inferior vena cava grafts in nude rats. Graft patency was maintained with no thrombus formation, followed by complete replacement of host ECs. Our study lays the foundation for future production of fully biologic TEVCs composed of hiPSC-derived ECs as an innovative therapy for SVCHDs.