JBRA assisted reproduction · 2026
The influence of melatonin supplementation on in vitro culture of murine embryos from polycystic ovary syndrome experimental models.
СтатьяНазвание и аннотация: Перевод в очереди
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Pinheiro Marcela de Oliveira, Cordeiro Fernanda Bertuccez, Ramos Giuliana Camila Ramirez, Moriyama Dóris Ferreira, Moraes Gabrielle Ferrante Alves de, Melo Karla Pacheco de, Trotta Maurício de Rosa, Ferreira Fernando Prado, Turco Edson Guimarães Lo
Аннотация
OBJECTIVE: Polycystic ovary syndrome (PCOS) negatively impacts oocyte and embryo quality. However, melatonin supplementation in assisted reproduction may enhance oocyte and embryo quality. This study aimed to analyze the effect of melatonin supplementation in embryo culture, by assessing embryos quality and development in murine models.
METHODS: C57BL/6J mice strain were divided into 6 groups: PCOS; PCOS with melatonin; placebo; placebo with melatonin; controls; and controls with melatonin. Embryo classification was performed during all developmental phases. TUNEL assay analysis was carried out in blastocysts.
RESULTS: The melatonin supplementation showed a positive influence during cleavage and morula development for all groups. The blastocyst rate was lower in the PCOS group when compared to placebo and controls. For the TUNEL assay, placebo and control groups supplemented with melatonin had a lower number of apoptotic cells compared to their respective non-supplemented groups.
CONCLUSIONS: Melatonin supplementation exerts a beneficial impact on cleavage and morula development for all groups. For PCOS, the poor blastocyst quality and high apoptosis rate emphasizes the inherent challenges associated with this condition. Melatonin potentially mitigates apoptotic events during early embryo development, suggesting its relevance as a supplementary therapeutic approach.
METHODS: C57BL/6J mice strain were divided into 6 groups: PCOS; PCOS with melatonin; placebo; placebo with melatonin; controls; and controls with melatonin. Embryo classification was performed during all developmental phases. TUNEL assay analysis was carried out in blastocysts.
RESULTS: The melatonin supplementation showed a positive influence during cleavage and morula development for all groups. The blastocyst rate was lower in the PCOS group when compared to placebo and controls. For the TUNEL assay, placebo and control groups supplemented with melatonin had a lower number of apoptotic cells compared to their respective non-supplemented groups.
CONCLUSIONS: Melatonin supplementation exerts a beneficial impact on cleavage and morula development for all groups. For PCOS, the poor blastocyst quality and high apoptosis rate emphasizes the inherent challenges associated with this condition. Melatonin potentially mitigates apoptotic events during early embryo development, suggesting its relevance as a supplementary therapeutic approach.