Dose-response : a publication of International Hormesis Society · 2026
High-Throughput Automated Construction and Validation of Dicentric Chromosome and Micronucleus Biodosimetry Calibration Curves for Radiation Emergency Response.
СтатьяНазвание и аннотация: Перевод в очереди
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Wu Mengyun, Li Haojun, Zhang Huadong, Li Wei, Gu Yeqing
Аннотация
OBJECTIVES: Develop a high-throughput, fully automated workflow for dicentric chromosome and micronucleus assays to enable rapid, accurate radiation dose estimation in radiological or nuclear emergencies.
METHODS: Blood from four healthy donors was irradiated (0-5.00 Gy, 137Cs γ-rays), repaired for 2 h at 37°C, and processed via an integrated platform (Chromprep + Metafer 4 + LS-CAS). Dicentric chromosomes (per 100 cells) and micronucleus (per 1,000 cells) were automatically scored. Dose-response data fitted to a quadratic model and validated using blinded samples with 95% confidence intervals accounting for Poisson statistics and curve uncertainty.
RESULTS: The automated dose-response curves for chromosome aberrations and micronuclei are y=8.4642x2-3.7786x+1.5439 and y=23.348x2+60.469x-1.6856, respectively. Calibration curves showed excellent fit (R 2=0.997 fordicentric chromosome; R 2=0.996 formicronucleus). All validation doses fell within estimated confidence intervals. The system achieved end-to-end automation with minimal human intervention while maintaining high accuracy and reproducibility.
CONCLUSION: This workflow provides a standardized, scalable, and reliable solution for rapid biodosimetry in mass-casualty radiological incidents, effectively bridging laboratory capacity and real-world emergency response needs.
METHODS: Blood from four healthy donors was irradiated (0-5.00 Gy, 137Cs γ-rays), repaired for 2 h at 37°C, and processed via an integrated platform (Chromprep + Metafer 4 + LS-CAS). Dicentric chromosomes (per 100 cells) and micronucleus (per 1,000 cells) were automatically scored. Dose-response data fitted to a quadratic model and validated using blinded samples with 95% confidence intervals accounting for Poisson statistics and curve uncertainty.
RESULTS: The automated dose-response curves for chromosome aberrations and micronuclei are y=8.4642x2-3.7786x+1.5439 and y=23.348x2+60.469x-1.6856, respectively. Calibration curves showed excellent fit (R 2=0.997 fordicentric chromosome; R 2=0.996 formicronucleus). All validation doses fell within estimated confidence intervals. The system achieved end-to-end automation with minimal human intervention while maintaining high accuracy and reproducibility.
CONCLUSION: This workflow provides a standardized, scalable, and reliable solution for rapid biodosimetry in mass-casualty radiological incidents, effectively bridging laboratory capacity and real-world emergency response needs.