Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA · 2003
[L-arginine decreases P-selectin expression in traumatic shock].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Huang Zong-hai, Lin Hong-wu, Li Zhou, Feng Hao-miao, Sun Ying-gang, Zhang Qing-guo
Аннотация
OBJECTIVE: To investigate the changes of P-selectin distribution in the vital organs and plasma during traumatic shock and explore the significance of these changes.
METHODS: Twenty-four normal SD rats were randomly assigned into 3 groups (n=8), namely traumatic shock group, L-arginine (L-Arg) treatment and control group. The rats in the former 2 groups were subjected to traumatic shock with L-Arg treatment group given 100 mg/kg L-Arg during resuscitation while the other receiving no medication. The control group received only intubation without trauma or phlebotomy. P-selectin expression in the vital organs including the heart, lungs, spleen, liver and small intestine was determined by means of streptavidin-biotin (SABC) immunocytochemical staining technique and serum P-selectin level assayed by enzyme-linked immunosorbent assay.
RESULTS: Before traumatic shock, P-selectin was scarcely detected in the vital organs except the lungs, and the serum was positive for P-selectin expression. Four hours after shock, intense P-selectin expression was observed in almost all the vital organs and the serum P-selectin level was significantly higher than that of the control group. In L-Arg treatment group, P-selectin levels were significantly lowered than those of shock group.
CONCLUSIONS: P-selectin expression in the vital organs and serum is up-regulated during traumatic shock in rats, possibly due to severe microcirculatory disorder and endothelial dysfunction in this condition. L-Arg may decrease the expression of P-selectin very likely through promoting endothelial NO synthesis.
METHODS: Twenty-four normal SD rats were randomly assigned into 3 groups (n=8), namely traumatic shock group, L-arginine (L-Arg) treatment and control group. The rats in the former 2 groups were subjected to traumatic shock with L-Arg treatment group given 100 mg/kg L-Arg during resuscitation while the other receiving no medication. The control group received only intubation without trauma or phlebotomy. P-selectin expression in the vital organs including the heart, lungs, spleen, liver and small intestine was determined by means of streptavidin-biotin (SABC) immunocytochemical staining technique and serum P-selectin level assayed by enzyme-linked immunosorbent assay.
RESULTS: Before traumatic shock, P-selectin was scarcely detected in the vital organs except the lungs, and the serum was positive for P-selectin expression. Four hours after shock, intense P-selectin expression was observed in almost all the vital organs and the serum P-selectin level was significantly higher than that of the control group. In L-Arg treatment group, P-selectin levels were significantly lowered than those of shock group.
CONCLUSIONS: P-selectin expression in the vital organs and serum is up-regulated during traumatic shock in rats, possibly due to severe microcirculatory disorder and endothelial dysfunction in this condition. L-Arg may decrease the expression of P-selectin very likely through promoting endothelial NO synthesis.