[Study on the pharmacological mechanism of analgesic effect of injection stauntoniae].

OBJECTIVE

To study the effects of Injection Stauntoniae (IS) on voltage-gated sodium currents in dorsal root ganglion neurons and analyze its pharmacological mechanism of blocking the nerve conduction and anal gesic action.

METHODS

Whole-cell patch-clamp recordings were performed in acutely isolated rat dorsal root ganglion neurons and the effects of 10% , 25% and 50 % IS on voltage-gated sodium currents were observed.

RESULTS

IS inhibited the peak sodium currents in dorsal root ganglion neurons in a dose-dependent way and affected the activation and inactivation process of the channels.

CONCLUSION

The analgesic effect of IS was presumably caused by modulation of voltage-gated sodium channels in primary sensory neurons besides structure destruction of myelin sheath and axon membrane.