Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics · 2026
[Susceptibility to seizures and expression of fibroblast growth factor 17 in rats with Cortical dysplasia and its effects on the hippocampal neurons].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Liu Tiantian, Liu Hengfang, Jia Yanjie
Аннотация
OBJECTIVE: To assess the susceptibility to seizures, expression of fibroblast growth factor 17 (Fgf17) in rats with Cortical dysplasia (CD), and its effect on hippocampal neurons.
METHODS: Pregnant SPF SD rats were intraperitoneally injected with Carmustine on E17 to establish a model of CD in the offspring rats. The offspring were randomly divided into the normal control, epilepsy group, CD group, and CD plus epilepsy group (n = 20 each). At 6 weeks of age, the rats from the epilepsy and CD plus epilepsy group were intraperitoneally injected with lithium chloride and pilocarpine to induce status epilepticus (SE). Quantitative real-time PCR and Western blotting were used to detect the mRNA and protein levels of Fgf17 at 24 hours and 30 days post-SE, respectively. An electrophysiological signal system was used to record the firing rate, amplitude, and seizure duration of the electroencephalogram (EEG) in rats from the 4 groups at 30 days post-SE. According to the concentration of Fgf17 in the culture medium, 12 1-day-old rats were divided into the control group, 500 nmol/L Fgf17 group, and 1 000 nmol/L Fgf17 group (n = 4 each). Immunofluorescence staining was used to observe the morphometry and number of hippocampal neurons in the 3 groups. This study was approved by the Ethics Committee of the Fifth Affiliated Hospital of Zhengzhou University (Ethics No.: KY2023005).
RESULTS: Hematoxylin-Eosin (HE) staining indicated CD in rats from the CD group and CD plus epilepsy group. Compared with the epilepsy group, the CD plus epilepsy group exhibited a faster rate of seizure onset, higher EEG amplitudes, and longer seizure at 30 days post-SE (P < 0.05). Both the CD and the CD plus epilepsy groups showed lower mRNA and protein expressions of Fgf17 in the hippocampus at 24 hours and 30 days post-SE compared with the control and epilepsy groups (P < 0.05), with the expression significantly decreased in the CD plus epilepsy group. Both the 500 nmol/L and the 1 000 nmol/L Fgf17 groups showed a greater number of hippocampal neurons compared with the control group (P < 0.05). The number of hippocampal neurons in the 1 000 nmol/L Fgf17 group was significantly greater compared with the 500 nmol/L Fgf17 group (P < 0.05).
CONCLUSION: Rats with CD exhibit decreased Fgf17 expression and increased epileptogenic susceptibility, which may be associated with reduced number of hippocampal neurons.
METHODS: Pregnant SPF SD rats were intraperitoneally injected with Carmustine on E17 to establish a model of CD in the offspring rats. The offspring were randomly divided into the normal control, epilepsy group, CD group, and CD plus epilepsy group (n = 20 each). At 6 weeks of age, the rats from the epilepsy and CD plus epilepsy group were intraperitoneally injected with lithium chloride and pilocarpine to induce status epilepticus (SE). Quantitative real-time PCR and Western blotting were used to detect the mRNA and protein levels of Fgf17 at 24 hours and 30 days post-SE, respectively. An electrophysiological signal system was used to record the firing rate, amplitude, and seizure duration of the electroencephalogram (EEG) in rats from the 4 groups at 30 days post-SE. According to the concentration of Fgf17 in the culture medium, 12 1-day-old rats were divided into the control group, 500 nmol/L Fgf17 group, and 1 000 nmol/L Fgf17 group (n = 4 each). Immunofluorescence staining was used to observe the morphometry and number of hippocampal neurons in the 3 groups. This study was approved by the Ethics Committee of the Fifth Affiliated Hospital of Zhengzhou University (Ethics No.: KY2023005).
RESULTS: Hematoxylin-Eosin (HE) staining indicated CD in rats from the CD group and CD plus epilepsy group. Compared with the epilepsy group, the CD plus epilepsy group exhibited a faster rate of seizure onset, higher EEG amplitudes, and longer seizure at 30 days post-SE (P < 0.05). Both the CD and the CD plus epilepsy groups showed lower mRNA and protein expressions of Fgf17 in the hippocampus at 24 hours and 30 days post-SE compared with the control and epilepsy groups (P < 0.05), with the expression significantly decreased in the CD plus epilepsy group. Both the 500 nmol/L and the 1 000 nmol/L Fgf17 groups showed a greater number of hippocampal neurons compared with the control group (P < 0.05). The number of hippocampal neurons in the 1 000 nmol/L Fgf17 group was significantly greater compared with the 500 nmol/L Fgf17 group (P < 0.05).
CONCLUSION: Rats with CD exhibit decreased Fgf17 expression and increased epileptogenic susceptibility, which may be associated with reduced number of hippocampal neurons.