Revista Brasileira de terapia intensiva · 2011
Molecular panel for detection of sepsis-related microorganisms.
СтатьяНазвание и аннотация: Перевод в очереди
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Ferreira Leslie Ecker, Dalposso Karilene, Hackbarth Bruna Barbosa, Gonçalves Anderson R, Westphal Glauco Adrieno, França Paulo Henrique Condeixa de, Pinho Mauro de Souza Leite
Аннотация
INTRODUCTION: Sepsis is a systemic inflammatory response related to high mortality rates in the hospital environment. Delayed etiological diagnosis and inadequate antimicrobial therapy are associated with treatment failures. Molecular tests based on polymerase chain reaction are regarded as faster and more accurate procedures than culture techniques for microbial identification, providing a higher rate of therapeutic success.
OBJECTIVE: To develop a panel of primers for DNA fragments of sepsis-related microorganisms.
METHODS: Primers for amplification of Enterobacter spp., Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida spp. were designed and tested for sensitivity and specificity on the basis of their respective standard strains.
RESULTS: The intended specificity was obtained for P. aeruginosa, S. aureus and Candida spp primers. Sensitivity tests showed a threshold for detection from 5 ng to 500 fg in blood samples contaminated with microbial DNA.
CONCLUSIONS: The molecular panel presented offers the advantage of a flexible 'open' system when compared to other multiplex detection methods.
OBJECTIVE: To develop a panel of primers for DNA fragments of sepsis-related microorganisms.
METHODS: Primers for amplification of Enterobacter spp., Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida spp. were designed and tested for sensitivity and specificity on the basis of their respective standard strains.
RESULTS: The intended specificity was obtained for P. aeruginosa, S. aureus and Candida spp primers. Sensitivity tests showed a threshold for detection from 5 ng to 500 fg in blood samples contaminated with microbial DNA.
CONCLUSIONS: The molecular panel presented offers the advantage of a flexible 'open' system when compared to other multiplex detection methods.