Lung · 2026
Sputum from Individuals with Primary Ciliary Dyskinesia Drives M2-like Macrophage Polarization.
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Wåhlander Jenny, Schmidt Tobias, Hansen Christine R, Kahn Robin, Påhlman Lisa I
Аннотация
INTRODUCTION: Primary ciliary dyskinesia (PCD) is a rare, congenital condition in which impaired ciliary function leads to bronchiectasis and progressive lung function decline. Bronchiectasis development is believed to involve infection and inflammation but is incompletely understood. Macrophages play a central role in cellular immune response, contributing to both pathogen clearance and immunoregulation. Depending on local stimuli, macrophages are polarized towards pro-inflammatory (M1) or pro-resolution/phagocytic (M2) phenotypes. This study aims to investigate the effects of PCD sputum on macrophage polarization.
METHODS: Sputum from 27 individuals with PCD and seven healthy controls were used to stimulate healthy monocyte-derived macrophages. Macrophage polarization was determined by surface markers, phagocytic ability and cytokine production using flow cytometry and immunoassays.
RESULTS: Macrophages stimulated with PCD sputum exhibited enhanced phagocytosis (MFI 194268 vs. 58235, p = 0.0002), increased expression of M2-associated surface markers CD163, CD206 and CD16, and reduced secretion of proinflammatory cytokines IL-6 (10.38 vs. 113.22 pg/ml, p = 0.0013) and IL-1β (0.75 vs. 3.60 pg/ml, p < 0.0001). Concurrently, expressions of M1-associated surface markers CD40 and CD80 were reduced.
CONCLUSION: PCD sputum induced a phagocytosis prone, M2-like phenotype in healthy macrophages.
METHODS: Sputum from 27 individuals with PCD and seven healthy controls were used to stimulate healthy monocyte-derived macrophages. Macrophage polarization was determined by surface markers, phagocytic ability and cytokine production using flow cytometry and immunoassays.
RESULTS: Macrophages stimulated with PCD sputum exhibited enhanced phagocytosis (MFI 194268 vs. 58235, p = 0.0002), increased expression of M2-associated surface markers CD163, CD206 and CD16, and reduced secretion of proinflammatory cytokines IL-6 (10.38 vs. 113.22 pg/ml, p = 0.0013) and IL-1β (0.75 vs. 3.60 pg/ml, p < 0.0001). Concurrently, expressions of M1-associated surface markers CD40 and CD80 were reduced.
CONCLUSION: PCD sputum induced a phagocytosis prone, M2-like phenotype in healthy macrophages.
Предметные рубрики MeSH
HumansSputumPhagocytosisFemaleAntigens, CDMacrophagesAdultMaleReceptors, Cell SurfaceReceptors, IgGLectins, C-TypeCD163 AntigenMannose ReceptorKartagener SyndromeAntigens, Differentiation, MyelomonocyticCase-Control StudiesPhenotypeMannose-Binding LectinsGPI-Linked ProteinsMiddle AgedYoung AdultCytokinesInterleukin-6Macrophage ActivationInterleukin-1beta