Clinical and experimental immunology · 1984
Presence of HLA-D/DR antigens on the membrane of breast tumour cells.
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Bernard D, Maurizis J C, Rusé F, Chassagne J, Chollet P, Sauvezie B, de Latour M, Plagne R
Аннотация
HLA-D/DR (Ia) glycoproteins were identified in human breast carcinoma and normal mammary gland cells by means of an anti-Ia monoclonal antibody. Two techniques were used: (1) immunoperoxidase staining performed on histological sections and (2) Ia glycoproteins were isolated as follows: firstly by radioactive labelling of isolated cells, then by filtration on Sephadex G25, followed by Lens culinaris chromatography, and immune complex formation and then elution on protein A-Sepharose. Lastly, the immune complex was studied by chromatofocusing. Both techniques revealed that Ia expression was found in carcinoma cells, but not in normal cells.