In vivo (Athens, Greece) · 2025
The Influence of Blood Glucose Instability on Aortic Health in Diabetes: Evaluating the Role of G-CSF Therapy.
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Jafri Ibrahim, Alsharif Ghadi, Al Zamzami Waseem, El-Sharnouby Mohamed, Abdel Monsef Ahmad S, Khalifa Mohamed M, Mehanna Osama M, Abbas Mohamed Ali Mahmoud, Sharahili Ahmed I, El Askary Ahmad
Аннотация
BACKGROUND/AIM: To evaluate the adverse effects of glucose fluctuations on the aorta in diabetic rats and to investigate the potential therapeutic role of granulocyte colony-stimulating factor (G-CSF).
MATERIALS AND METHODS: Thirty-nine adult male albino rats (10-12 weeks old, 150-180 g) were divided equally into three groups: diabetic, control, and Filgrastim (a human G-CSF)-treated. Diabetes was induced using alloxan in the diabetic and Filgrastim groups. The Filgrastim-treated group received intraperitoneal glucose injections every four hours for four days, followed by subcutaneous Filgrastim (200 μg/kg/day) for five days. Histological analysis of aortic sections was conducted using Hematoxylin and Eosin staining.
RESULTS: Significant differences were observed between groups in insulin levels (p=0.011), tunica intima thickness (p=0.004), tunica media thickness (p=0.000), and the proportions of collagen and smooth muscle fibers in the aortic wall (p<0.01). Diabetic rats exhibited increased endothelial damage and structural alterations in the aortic wall, while Filgrastim treatment mitigated these changes.
CONCLUSION: Glucose fluctuations caused more severe endothelial damage in the aorta of diabetic rats compared to controls. G-CSF shows promise as a potential therapy for cardiovascular complications associated with fluctuating hyperglycemia.
MATERIALS AND METHODS: Thirty-nine adult male albino rats (10-12 weeks old, 150-180 g) were divided equally into three groups: diabetic, control, and Filgrastim (a human G-CSF)-treated. Diabetes was induced using alloxan in the diabetic and Filgrastim groups. The Filgrastim-treated group received intraperitoneal glucose injections every four hours for four days, followed by subcutaneous Filgrastim (200 μg/kg/day) for five days. Histological analysis of aortic sections was conducted using Hematoxylin and Eosin staining.
RESULTS: Significant differences were observed between groups in insulin levels (p=0.011), tunica intima thickness (p=0.004), tunica media thickness (p=0.000), and the proportions of collagen and smooth muscle fibers in the aortic wall (p<0.01). Diabetic rats exhibited increased endothelial damage and structural alterations in the aortic wall, while Filgrastim treatment mitigated these changes.
CONCLUSION: Glucose fluctuations caused more severe endothelial damage in the aorta of diabetic rats compared to controls. G-CSF shows promise as a potential therapy for cardiovascular complications associated with fluctuating hyperglycemia.