Generation of an induced pluripotent stem cell line (AHMUCNi004-A) from a 14-year-old male with Down syndrome.

Down syndrome (DS), resulting from trisomy 21, is the most prevalent genetic cause of neurodevelopmental impairment and a significant risk factor for developing pathology of Alzheimer's disease (AD). In this study, we generated an induced pluripotent stem cell (iPSC) line (AHMUCNi004-A) from peripheral blood mononuclear cells (PBMCs) of a 14-year-old male with DS using non-integrative episomal vectors. The AHMUCNi004-A line exhibits typical iPSCs' morphology, expresses hallmark pluripotency markers, and maintains the constitutive trisomy 21 karyotype. Furthermore, the line demonstrates the capacity to differentiate into derivatives of all three embryonic germ layers.