Detrimental effect of hydrosalpinx fluid on the development and blastulation of mouse embryos in vitro.

OBJECTIVE

To study the effects of human hydrosalpinx fluid on mouse embryo blastulation rate.

DESIGN

Comparison of mouse embryo blastulation rate in media containing increasing concentrations of hydrosalpinx fluid.

SETTING

Tertiary care center.

PATIENT(S): Women undergoing laparoscopic evaluation or treatment for infertility noted to have hydrosalpinx or paratubal cyst.

INTERVENTION(S): Exposure of mouse embryos to hydrosalpinx or paratubal cyst fluid collected during laparoscopy.

MAIN OUTCOME MEASURE(S): Blastulation rate of mouse embryos.

RESULT(S): Culture of mouse embryos at 0% (controls), 0.3%, 0.6%, and 0.9% hydrosalpinx fluid concentrations demonstrated significantly lower blastulation rate at each level compared with the controls.

CONCLUSION(S): Hydrosalpinx fluid is highly embryotoxic.