The Association of the Polymorphic Marker of +795 G>A in the Adiponectin Receptor 2 Gene with Biopsy-confirmed Metabolic Dysfunction-associated Steatotic Liver Disease.

BACKGROUND AND AIMS: Metabolic dysfunction-associated steatotic liver disease (MASLD) with the prevalence of around 25% is the most prevailing cause of chronic liver disease. In this study, we aimed to investigate the association of the rs16928751 or +795 G>A variant in adiponectin receptor 2 gene (ADIPOR2) with MASLD.

METHODS

Genomic DNA was isolated from the whole blood of 130 patients with biopsy-confirmed MASLD, and 130 controls according to the phenol-chloroform extraction and ethanol precipitation approach. Then the polymorphic marker of +795 G>A was genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTS

The +795 G>A variant met the Hardy-Weinberg equilibrium (p > 0.05) in both control and patient groups; hence, the samples had good population representativeness. The genotype count of ADIPOR2 gene +795 G>A differed significantly between these two groups. The +795 G>A "AA" genotype in comparison to the "GG" was more frequent in the patients with MASLD (p=0. 037; OR=2.24, 95%CI: 1.20-6.47).

CONCLUSIONS

To our knowledge, this study is the first one that found a significant association between the +795 G>A variant of the ADIPOR2 gene and biopsy-confirmed MASLD; nonetheless, it needs to be corroborated by further research in different populations.