Biochimica et biophysica acta. Biomembranes · 2022
Ts17, a Tityus serrulatus β-toxin structurally related to α-scorpion toxins.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Menezes Luis Felipe Santos, Maranhão Mariza Mendanha, Tibery Diogo Vieira, de Souza Adolfo Carlos Barros, da Mata Daniel Oliveira, Campos Leandro Ambrósio, Souza Amanda Araújo, Freitas Sonia Maria de, Schwartz Elisabeth Ferroni
Аннотация
Ts17 was purified from the venom of the scorpion Tityus serrulatus, the most dangerous scorpion species in Brazil. The activity on Nav1.1-Nav1.7 channels was electrophysiologically characterized by patch-clamp technique. Ts17 amino acid sequence indicated high similarity to alpha-scorpion toxins; however, it presented beta-toxin activity, altering the kinetics of the Na+-channels. The most affected subtypes during activation (with and without prepulse) and inactivation phases were Nav1.2 and Nav1.5, respectively. For recovery from inactivation, the most affected voltage-gated sodium channel was Nav1.5. Circular dichroism spectra showed that Ts17 presents mainly β-sheet and unordered structures at all analyzed pHs, and the maximum value of α-helix was found at pH 4.0 (13.3 %). Based on the results, Ts17 might be used as a template to develop a new cardiac drug. Key contribution Purification of Ts17 from Tityus serrulatus, electrophysiological characterization of Ts17 on voltage-gated sodium channel subtypes, β-toxin classification.