Regulation of oviduct function in pigs by local transfer of ovarian steroids and prostaglandins: a mechanism to influence sperm transport.

Blood was collected from a prominent ear vein and from the arterioles supplying the caudal portion of each oviduct in 5 estrous animals. Sampling was arranged at 4 preovulatory intervals, and just after ovulation in the fifth animal. Steroid hormones and prostaglandins were determined by radioimmunoassay of plasma. Whereas the concentration of cortisol did not differ between peripheral and oviduct blood samples, the overall values for progesterone, androstenedione and estradiol were significantly higher in oviduct samples (P less than 0.001) - frequently by 5- to 10-fold or more. The concentration of these three hormones in oviduct blood corresponded, in most instances, with the number of preovulatory follicles or recent ovulations in the adjacent ovary but suitable statistical correlations could not be performed. Prostaglandin F2 alpha was also present in higher concentrations in oviduct compared with peripheral plasma (P less than 0.005), although pulmonary clearance of prostaglandins would have influenced the peripheral values. Differences in the concentration of prostaglandin E2 were not significant. A local transfer of follicular hormones from the ovarian vein to the ovarian and utero-tubal arteries is proposed as a means of regulating oviduct function. Elevated concentrations of steroids and prostaglandins transferred in this way would permit incisive changes in the patency and contractile activity of the isthmus, thereby facilitating the periovulatory phase of sperm transport from the caudal portion of the oviduct to the site of fertilization.