[Mechanisms of stem cell-derived extracellular vesicles protecting Leydig cells from acute oxidative injury in rats].

OBJECTIVE

To investigate the mechanisms of stem cell-derived extracellular vesicles (EVs) alleviating acute oxidative stress injury to Leydig cells.

METHODS

The model of testicular ischemia-reperfusion was established by unilateral testicular resection in 10 SD rats followed by injection of EVs into the tail vein in 5 of the animals (the experimental group) and that of isotonic PBS in the other 5(the control group).At 2 hours after reperfusion, the infiltration of neutrophilsin the testicular tissue was detected by HE staining, the apoptosis of Leydig cells measured by TUNEL and Caspase-3 immunohistochemistry, and the proliferation of Leydig cellsdetermined by Ki-67 immunohistochemical staining.

RESULTS

Compared with the control, the experimental group showed significantlymilderinfiltration of neutrophils in the interstitial tissueof the testis and lower apoptosis and higher proliferation of Leydig cells.

CONCLUSIONS

Stem cell EVs can reduce the adhesion of neutrophils in the acute phase and alleviate acute oxidative stress injury to Leydig cells by decreasing the production of reactive oxygen free radicals.