Analysis of Salicylic and Phenolic Acids in the Plant by HPLC-Fluorescence Detector.

Salicylic acid is a member of benzoic acid derivatives, a group of compounds which have a backbone of C6C1 consisting of one carboxyl group and one (or more) hydroxyl group(s) attached to the aromatic ring. Salicylic acid is a signaling compound in systemic acquired resistance (SAR). An increased level of salicylic acid is found in the plant after a fungi's attack, which further induces the accumulation of phytoalexins, low molecular weight defense compounds. Here, we describe the extraction method and determination of salicylic acid using high-performance liquid chromatography (HPLC) coupled with fluorescence detection.