Bioanalysis · 2026
Rapid quantification of bile acids in serum by LC-MS/MS and application to serum bile acid profile in voriconazole administered patients with invasive fungal infections.
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Yang Xuping, Qin Haoran, Ling Jing, Dong Lulu, Jiang Yan, Zou Sulan, Hu Nan
Аннотация
OBJECTIVES: Disruption of bile acid homeostasis is implicated in the pathogenesis and progress of several liver diseases, including drug-induced liver injury (DILI). A rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying 30 bile acids in human serum was developed and applied to analyze serum bile acid profile in voriconazole administered patients with invasive fungal infections.
METHODS: The stable isotope-coded bile acids were used as internal standards. The analytes in serum samples (50 μL) were extracted by protein precipitation and isolated by a Kinetex EVO C18 column (50 × 2.1 mm, 2.6 μm). The detection was performed using multiple reaction monitoring (MRM) in electrospray negative ionization mode.
RESULTS: All of the 30 bile acids were sufficiently separated within 10 min with good linearity (r > 0.99) over tested calibration ranges. The accuracy and precision values were in the range of 85.42-114.3% and 1.8-13.8%, respectively. The matrix effect, recovery, stability, and dilution integrity all met the acceptance criteria. Serum bile acid profile changed in patients with supratherapeutic voriconazole concentration (>10 μg/mL).
CONCLUSIONS: This LC-MS/MS method for the quantification of 30 bile acids in human serum might apply a new analytical approach for liver function evaluation.
METHODS: The stable isotope-coded bile acids were used as internal standards. The analytes in serum samples (50 μL) were extracted by protein precipitation and isolated by a Kinetex EVO C18 column (50 × 2.1 mm, 2.6 μm). The detection was performed using multiple reaction monitoring (MRM) in electrospray negative ionization mode.
RESULTS: All of the 30 bile acids were sufficiently separated within 10 min with good linearity (r > 0.99) over tested calibration ranges. The accuracy and precision values were in the range of 85.42-114.3% and 1.8-13.8%, respectively. The matrix effect, recovery, stability, and dilution integrity all met the acceptance criteria. Serum bile acid profile changed in patients with supratherapeutic voriconazole concentration (>10 μg/mL).
CONCLUSIONS: This LC-MS/MS method for the quantification of 30 bile acids in human serum might apply a new analytical approach for liver function evaluation.