Synchronous changes in mercury stable isotopes and compound-specific amino acid nitrogen isotopes in organisms through food chains.

The relationship between stable isotope of mercury (Hg, Δ199Hg and δ202Hg) and compound-specific nitrogen isotope of amino acids (CSIA-AA, δ15NGlu and δ15NPhe) remains poorly understood. In this study, we investigated bird species and their prey in an abandoned Hg mining area, southern China to elucidate these correlations for a better understanding of Hg sources, biological transfer, accumulation and amplification through food chains. Our findings revealed distinct isotopic patterns: Δ199Hg showed a positive correlation with δ15NGlu, indicating trophic transfer processes, while a negative correlation with δ15NPhe suggested differences in Hg sources among birds. The wide ranges of δ15NPhe and Δ199Hg observed in birds appear to reflect mixtures of multiple nitrogen and Hg sources, likely due to their diverse food sources and the large variation in the proportion of MeHg in total Hg (MeHg%). The consistent slope between Δ199Hg/δ15Nphe and MeHg%/δ15Nphe, reflecting both energy and Hg sources, provides new insights into the biotransfer and accumulation of Hg in organisms. Notably, the trophic magnification factor (TMF) of MeHg observed in water birds, such as egrets, reached an exceptionally high value of 97.7 estimated from CSIA of multiple amino acids (i.e., TMFM), underscoring the significance of investigating Hg sources in birds. Our results demonstrate that the synchronous changes between CSIA-AA and odd Hg isotopes effectively identify Hg sources and transfer across multiple ecological systems.