A Novel Skeleton Compound from Streptomyces canarius SN0246: Isolation, Purification, and Structural Elucidation.

In this study, a compound with a novel chemical skeleton was isolated and purified from the fermentation broth of Streptomyces canarius SN0246. Its structure was elucidated using nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HRMS), and the compound was named canakingmycin. The in vitro antifungal activity of canakingmycin was evaluated against common crop pathogenic fungi, Rhizoctonia solani. The bioassay results showed that canakingmycin exhibited notable inhibitory effects on the mycelial growth of the phytopathogen, with the EC50 value of 76.13 μg/mL. This study enriches the structural diversity of secondary metabolites from Streptomyces and provides a promising lead molecule and scientific basis for the development of new bio-based fungicides.