Phytomedicine : international journal of phytotherapy and phytopharmacology · 2026
Chemical proteomics identifies an autophagy receptor as a functional target of Shikonin in enhancing chemotherapy.
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Yuan Meng, Xu Zhipeng, Tian Danmei, Wang Fangfang, Xia Zixuan, Zhang Bei, Zhang Youwei, Tang Jinshan
Аннотация
BACKGROUND: Shikonin (SKN), a dihydroxy-1,4-naphthoquinone extracted from the roots of Lithospermum spp, exhibited diverse pharmacological activities, including potent antitumor effects. Our previous work demonstrated that SKN enhanced chemosensitivity by suppressing activation of the DNA damage response (DDR) pathway through autophagy-dependent degradation of the ATM kinase; however, its direct molecular targets remained unidentified.
PURPOSE: This study aimed to identify the cellular targets of SKN and elucidate the molecular mechanisms underlying its chemosentizing activity.
METHODS: To identify SKN-associated cellular targets, we employed activity-based protein profiling (ABPP) combined with functional validation. SKN-derived probes were developed to enable chemoproteomic target identification using LC-MS/MS followed by bioinformatic analysis. Interactions between SKN and candidate proteins were validated using pull-down assays coupled with western blotting, cellular thermal shift assay (CETSA), and co-immunoprecipitation. Functional roles of identified targets were further examined using RNA interference (RNAi) and overexpression approaches.
RESULTS: Six SKN-derived probes were developed with minimal modifications to support chemoproteomic target identification while maintaining the native activity of shikonin. Chemoproteomic profiling identified SQSTM1/P62, an autophagy cargo receptor, as a critical mediator of SKN-induced chemosensitization. Functional validation revealed that modulation of SQSTM1/P62 markedly affected SKN-induced ATM degradation and the associated enhancement of chemosensitivity.
CONCLUSION: This study reveals a previously unrecognized molecular mechanism underlying the chemosensitizing effects of SKN and establishes a target-informed strategy for identifying direct cellular targets of natural products. These findings provide a mechanistic basis for the development of SKN-based combination strategies in cancer therapy.
PURPOSE: This study aimed to identify the cellular targets of SKN and elucidate the molecular mechanisms underlying its chemosentizing activity.
METHODS: To identify SKN-associated cellular targets, we employed activity-based protein profiling (ABPP) combined with functional validation. SKN-derived probes were developed to enable chemoproteomic target identification using LC-MS/MS followed by bioinformatic analysis. Interactions between SKN and candidate proteins were validated using pull-down assays coupled with western blotting, cellular thermal shift assay (CETSA), and co-immunoprecipitation. Functional roles of identified targets were further examined using RNA interference (RNAi) and overexpression approaches.
RESULTS: Six SKN-derived probes were developed with minimal modifications to support chemoproteomic target identification while maintaining the native activity of shikonin. Chemoproteomic profiling identified SQSTM1/P62, an autophagy cargo receptor, as a critical mediator of SKN-induced chemosensitization. Functional validation revealed that modulation of SQSTM1/P62 markedly affected SKN-induced ATM degradation and the associated enhancement of chemosensitivity.
CONCLUSION: This study reveals a previously unrecognized molecular mechanism underlying the chemosensitizing effects of SKN and establishes a target-informed strategy for identifying direct cellular targets of natural products. These findings provide a mechanistic basis for the development of SKN-based combination strategies in cancer therapy.