Journal of applied microbiology · 2026
Enrichment and characterization of microbial consortium XY04 with high phenol tolerance and biodegradation capacity.
СтатьяНазвание и аннотация: Перевод в очереди
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Zhao Wei, Wang Bin, Zhao Chenfei, Shi Liuyang, Han Yifan, Wang Jingjing, Xu Song, Zhang Xiaoxia, Yu Chan, Hu Linzhen, Wang Xingbiao, Huang Zhiyong
Аннотация
AIMS: This study aims to obtain a highly efficient and tolerant microbial community for the treatment of high-concentration phenolic and formaldehyde wastewater and analyze its community response mechanisms to high-concentration pollution.
METHODS AND RESULTS: Using acclimation techniques, we developed microbial consortium XY04, capable of degrading up to 3000 mg L-1 phenol and 2000 mg L-1 formaldehyde. XY04 degraded 1500 mg L-1 phenol within 24 h (62.5 mg L-1·h-1) and 1500 mg L-1 formaldehyde within 12 h (125 mg L-1·h-1). The consortium maintained high degradation efficiency across a wide range of conditions (pH 3-9, salinity 0%-3%, 20-35°C), and retained activity under extreme conditions (5% salinity, pH 11). Substrate spectrum analysis showed metabolic capacity for phenols, aldehydes, and aromatic hydrocarbons, with enhanced degradation efficiency from moderate carbon source supplementation. Community analysis revealed compositional shifts under high- and low-concentration conditions, with functional prediction indicating enhanced genes for phenol meta-cleavage and carbon, amino acid, and lipid metabolism under stress. ASV (amplicon sequence variant)-function coupling analysis showed that amplicon sequence variants enriched in high-concentration environments contributed to these pathways, suggesting community restructuring for stress adaptation.
CONCLUSIONS: A highly efficient microbial consortium XY04 was obtained for phenolic and formaldehyde wastewater treatment, exhibiting tolerance to various environmental conditions and the ability to adapt to high-concentration pollution through community self-organization.
METHODS AND RESULTS: Using acclimation techniques, we developed microbial consortium XY04, capable of degrading up to 3000 mg L-1 phenol and 2000 mg L-1 formaldehyde. XY04 degraded 1500 mg L-1 phenol within 24 h (62.5 mg L-1·h-1) and 1500 mg L-1 formaldehyde within 12 h (125 mg L-1·h-1). The consortium maintained high degradation efficiency across a wide range of conditions (pH 3-9, salinity 0%-3%, 20-35°C), and retained activity under extreme conditions (5% salinity, pH 11). Substrate spectrum analysis showed metabolic capacity for phenols, aldehydes, and aromatic hydrocarbons, with enhanced degradation efficiency from moderate carbon source supplementation. Community analysis revealed compositional shifts under high- and low-concentration conditions, with functional prediction indicating enhanced genes for phenol meta-cleavage and carbon, amino acid, and lipid metabolism under stress. ASV (amplicon sequence variant)-function coupling analysis showed that amplicon sequence variants enriched in high-concentration environments contributed to these pathways, suggesting community restructuring for stress adaptation.
CONCLUSIONS: A highly efficient microbial consortium XY04 was obtained for phenolic and formaldehyde wastewater treatment, exhibiting tolerance to various environmental conditions and the ability to adapt to high-concentration pollution through community self-organization.