Journal of ethnopharmacology · 2026
Bioactive components of Mongolian medicinal Gentiana dahurica Fisch. flowers inhibit the NF-κB signaling pathway and ameliorate chronic obstructive pulmonary disease.
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Wang Ning, Zhang Wenxue, Li Jun, Bao Fushun, Feng Weihua, Yang Yan, Zhou Shuhong, Guo Qi, Song Wenxuan, Chen Xingyu, Ma Ao, Yi Letai, Wang Minjie
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Gentiana dahurica Fisch. is a traditional herb widely used in Chinese and Mongolian medicine for the management of respiratory disorders such as cough, sore throat, and "lung heat". Its historical application provides a strong rationale for investigating its potential in the management of COPD. Its application in Mongolian medicine for 'lung heat' syndrome provides a direct ethnopharmacological basis for exploring its anti-COPD potential.
AIM OF THE STUDY: This study primarily aimed to evaluate the anti-COPD effects of G. dahurica flowers and elucidate its underlying molecular mechanisms, with a particular focus on the regulatory effects of its bioactive components on inflammatory signaling pathways.
MATERIALS AND METHODS: Chemical profiling was performed using HPLC-Q-Exactive-MS. A laboratory-induced COPD model was established in mice using LPS and cigarette smoke extract (CSE), followed by a 12-week treatment with G. dahurica extract. Assessments included lung function tests, histopathology, qPCR, and proteomic analysis. In vitro validation was performed on LPS-stimulated cultures, involving two distinct lung cell types: human BEAS-2B and mouse MLE-12.
RESULTS: Fifty compounds were identified in the extract. Treatment significantly alleviated pulmonary inflammation and improved lung function in mice. Proteomic analysis revealed inhibition of the NF-κB signaling pathway. Additionally validated by downregulation of inflammatory genes. Isoorientin and luteolin were confirmed as key active compounds mediating these effects.
CONCLUSION: G. dahurica flowers ameliorate COPD progression via suppressing the NF-κB signaling pathway, which provides a solid molecular basis for its traditional application in the treatment of respiratory disorders. These findings suggest that G. dahurica flowers hold great potential as a promising therapeutic agent for COPD intervention.
AIM OF THE STUDY: This study primarily aimed to evaluate the anti-COPD effects of G. dahurica flowers and elucidate its underlying molecular mechanisms, with a particular focus on the regulatory effects of its bioactive components on inflammatory signaling pathways.
MATERIALS AND METHODS: Chemical profiling was performed using HPLC-Q-Exactive-MS. A laboratory-induced COPD model was established in mice using LPS and cigarette smoke extract (CSE), followed by a 12-week treatment with G. dahurica extract. Assessments included lung function tests, histopathology, qPCR, and proteomic analysis. In vitro validation was performed on LPS-stimulated cultures, involving two distinct lung cell types: human BEAS-2B and mouse MLE-12.
RESULTS: Fifty compounds were identified in the extract. Treatment significantly alleviated pulmonary inflammation and improved lung function in mice. Proteomic analysis revealed inhibition of the NF-κB signaling pathway. Additionally validated by downregulation of inflammatory genes. Isoorientin and luteolin were confirmed as key active compounds mediating these effects.
CONCLUSION: G. dahurica flowers ameliorate COPD progression via suppressing the NF-κB signaling pathway, which provides a solid molecular basis for its traditional application in the treatment of respiratory disorders. These findings suggest that G. dahurica flowers hold great potential as a promising therapeutic agent for COPD intervention.