Journal of global antimicrobial resistance · 2025
First report of chromosomal co-occurrence of blaAFM-3 and blaIMP-45 genes in a multidrug-resistant Pseudomonas aeruginosa clinical isolate.
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Zhong Yu-Xia, Cai Gui-Jun, Dai Li-Ting, Yang Ling, Chen Ding-Qiang, Yuan Pei-Bo
Аннотация
BACKGROUND: Multidrug-resistant (MDR) Pseudomonas aeruginosa, especially carbapenemase-producing strains, poses major clinical challenges due to their widespread dissemination and limited treatment options.
CASE AND METHODS: A 65-y-old patient with intracranial infection was hospitalized. An MDR P. aeruginosa strain isolated from her cerebrospinal fluid was analysed using MALDI-TOF mass spectrometry, antimicrobial susceptibility testing, and whole genome sequencing.
RESULTS: P. aeruginosa strain YB1 was resistant to carbapenems, cephalosporins, quinolones, and aminoglycosides, except colistin. Its genome comprised a 6.41-Mb chromosome and a 448-kb IncP-like plasmid. Six β-lactamase genes were identified on the chromosome: blaPER-1, blaOXA-1, blaIMP-45, and blaAFM-3 in the MDR region, with blaOXA-488 and blaPAO-201 located outside it. The chromosomal MDR region shared high homology with plasmid-borne Tn6485e, which originated from multiple plasmids. Specifically, the novel carbapenemase gene blaAFM-3 resided in a conserved transposon (ISCR27n3-groL-△floR-blaAFM-3-bleMBL-trpF-ISCR27n2), identical to that originally characterized in the Alcaligenes faecalis plasmid. Additionally, blaIMP-45 was located in a conserved type I integron (IntI-aac(6')-Ib-blaIMP-45-blaOXY-1-catB3-△qacE-sul1). Notably, the 3'-terminal sequence of Tn6485e (IS26-aph(3')-Ia-IS26-tet(C)-tetR(C)-IS26-IS6100), absent from the chromosomal MDR region, was found on YB1's plasmid. Moreover, IS26 flanked the 71 600-bp chromosomal fragment covering the MRD region, with a 14-bp inverted repeat (5'-GGCACTGTTGCAAA-3') on their outer sides in the same orientation, implying potential IS26-facilitated plasmid-chromosome recombination.
CONCLUSIONS: This study first identified the coexistence of the blaAFM-3 and blaIMP-45 genes on the chromosome of P. aeruginosa. Monitoring gene transfer from plasmids to chromosomes is crucial, as it boosts bacterial survival against antibiotics and enables heritable resistance, threatening human health.
CASE AND METHODS: A 65-y-old patient with intracranial infection was hospitalized. An MDR P. aeruginosa strain isolated from her cerebrospinal fluid was analysed using MALDI-TOF mass spectrometry, antimicrobial susceptibility testing, and whole genome sequencing.
RESULTS: P. aeruginosa strain YB1 was resistant to carbapenems, cephalosporins, quinolones, and aminoglycosides, except colistin. Its genome comprised a 6.41-Mb chromosome and a 448-kb IncP-like plasmid. Six β-lactamase genes were identified on the chromosome: blaPER-1, blaOXA-1, blaIMP-45, and blaAFM-3 in the MDR region, with blaOXA-488 and blaPAO-201 located outside it. The chromosomal MDR region shared high homology with plasmid-borne Tn6485e, which originated from multiple plasmids. Specifically, the novel carbapenemase gene blaAFM-3 resided in a conserved transposon (ISCR27n3-groL-△floR-blaAFM-3-bleMBL-trpF-ISCR27n2), identical to that originally characterized in the Alcaligenes faecalis plasmid. Additionally, blaIMP-45 was located in a conserved type I integron (IntI-aac(6')-Ib-blaIMP-45-blaOXY-1-catB3-△qacE-sul1). Notably, the 3'-terminal sequence of Tn6485e (IS26-aph(3')-Ia-IS26-tet(C)-tetR(C)-IS26-IS6100), absent from the chromosomal MDR region, was found on YB1's plasmid. Moreover, IS26 flanked the 71 600-bp chromosomal fragment covering the MRD region, with a 14-bp inverted repeat (5'-GGCACTGTTGCAAA-3') on their outer sides in the same orientation, implying potential IS26-facilitated plasmid-chromosome recombination.
CONCLUSIONS: This study first identified the coexistence of the blaAFM-3 and blaIMP-45 genes on the chromosome of P. aeruginosa. Monitoring gene transfer from plasmids to chromosomes is crucial, as it boosts bacterial survival against antibiotics and enables heritable resistance, threatening human health.