Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology · 2026
Evaluation of anti-desmoglein-2 antibodies and cardiac biomarkers in Boxer dogs with arrhythmogenic right ventricular cardiomyopathy.
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Dos Santos L, Cunningham S, Mantovani M
Аннотация
INTRODUCTION/OBJECTIVE: The aim of this study was to evaluate whether circulating anti-desmoglein-2 antibodies distinguish Boxer dogs with arrhythmogenic right ventricular cardiomyopathy from screened controls and assess the diagnostic utility of anti-desmoglein-2, cardiac troponin I (cTnI), high-sensitivity cTnI (HS-cTnI), and N-terminal pro-B-type natriuretic peptide (NT-proBNP) relative to arrhythmia burden.
ANIMALS, MATERIALS AND METHODS: This prospective cross-sectional study included 52 Boxer dogs (28 arrhythmogenic right ventricular cardiomyopathy-affected and 24 screened controls). Diagnosis was based on Holter monitoring (>300 ventricular premature complexes [VPCs]/24 h or documented ventricular tachycardia). Anti-desmoglein-2 antibodies, cTnI, HS-cTnI, and NT-proBNP were measured. Receiver operating characteristic analysis and correlations with VPC burden were performed.
RESULTS: Anti-desmoglein-2 antibody concentrations did not differ between groups by either method (enzyme-linked immunosorbent assay: P=0.687; Western blot: P=0.293). Receiver-operating characteristic analysis showed poor discriminatory ability for anti-desmoglein-2 (enzyme-linked immunosorbent assay: area under the receiver operating characteristic curve [AUC] = 0.534, P=0.693; Western blot: AUC = 0.600, P=0.295). In contrast, cutoffs for NT-proBNP (1530 pmol/L, AUC = 0.856), HS-cTnI (0.21 ng/mL, AUC = 0.805), and cTnI (0.08 ng/mL, AUC = 0.795) all demonstrated good performance in differentiating groups (P<0.001) and showed positive correlations with VPCs (P≤0.001) for NT-proBNP (r = 0.60), HS-cTnI (r = 0.549), and cTnI (r = 0.471).
LIMITATIONS: The cross-sectional design and lack of pedigree data may have limited detection of subclinical disease. Disease stage and therapy may also have influenced biomarker concentrations.
CONCLUSIONS: Anti-desmoglein-2 antibody lacked diagnostic value for arrhythmogenic right ventricular cardiomyopathy in Boxer dogs, whereas NT-proBNP, cTnI, and HS-cTnI showed promise as reliable clinical biomarkers.
ANIMALS, MATERIALS AND METHODS: This prospective cross-sectional study included 52 Boxer dogs (28 arrhythmogenic right ventricular cardiomyopathy-affected and 24 screened controls). Diagnosis was based on Holter monitoring (>300 ventricular premature complexes [VPCs]/24 h or documented ventricular tachycardia). Anti-desmoglein-2 antibodies, cTnI, HS-cTnI, and NT-proBNP were measured. Receiver operating characteristic analysis and correlations with VPC burden were performed.
RESULTS: Anti-desmoglein-2 antibody concentrations did not differ between groups by either method (enzyme-linked immunosorbent assay: P=0.687; Western blot: P=0.293). Receiver-operating characteristic analysis showed poor discriminatory ability for anti-desmoglein-2 (enzyme-linked immunosorbent assay: area under the receiver operating characteristic curve [AUC] = 0.534, P=0.693; Western blot: AUC = 0.600, P=0.295). In contrast, cutoffs for NT-proBNP (1530 pmol/L, AUC = 0.856), HS-cTnI (0.21 ng/mL, AUC = 0.805), and cTnI (0.08 ng/mL, AUC = 0.795) all demonstrated good performance in differentiating groups (P<0.001) and showed positive correlations with VPCs (P≤0.001) for NT-proBNP (r = 0.60), HS-cTnI (r = 0.549), and cTnI (r = 0.471).
LIMITATIONS: The cross-sectional design and lack of pedigree data may have limited detection of subclinical disease. Disease stage and therapy may also have influenced biomarker concentrations.
CONCLUSIONS: Anti-desmoglein-2 antibody lacked diagnostic value for arrhythmogenic right ventricular cardiomyopathy in Boxer dogs, whereas NT-proBNP, cTnI, and HS-cTnI showed promise as reliable clinical biomarkers.