Synthesis NMR characterization and X-ray structure of α,β-unsaturated steroid spiroketal lactones. evaluation of cytotoxicity and anti-inflammatory activity.

Aldol condensation between different steroid sapogenins and pyruvic acid in the presence of BF3·Et2O, led to steroids with a α,β-unsaturated spiroketal lactone side chain that produced significant reduction on the production of nitric oxide in LPS-stimulated macrophages, suggesting potential anti-inflammatory activity. One of the obtained compounds showed strong cytotoxicity against HeLa cervical cancer cells.'