The Journal of nutrition · 2025
The Diversity of Oligosaccharide Composition in Rabbit Milk and Its Association with the Development of the Cecal Microbiota and the Survival of Young Rabbits.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Combes Sylvie, Knudsen Christelle, Rumeau Mathilde, Pascal Géraldine, Cauquil Laurent, Helies Virginie, Ruesche Julien, Poli Maryse, Jones Elisabeth, Lencina Corinne, Castinel Adrien, Averty Estelle, Venot Eric, Cholet Sophie, Beaumont Martin, Fenaille François
Аннотация
BACKGROUND: Milk oligosaccharides (MOs) are involved in microbiota development and health in newborns. Given the physiological immaturity of rabbits at birth, we hypothesized that MO profile diversity could explain the variability of survival and microbiota composition in young rabbits.
OBJECTIVE: Our aims were as follows 1) to characterize the MOs in rabbit, 2) to assess the variability in MO profiles over the course of lactation, 3) to evaluate associations with young rabbit survival, and 4) to explore links with cecal microbiota composition.
METHODS: MOs from 76 rabbit does were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Changes over lactation were evaluated in a subset of 10 does. Young rabbit survival data were recorded across 7 reproductive cycles in 67 does. Cecal microbiota composition was assessed in 20 young rabbits at 8 and 26 d and correlated with maternal MO profiles.
RESULTS: A total of 49 MOs were identified, with 3 (sialyllactose, Hex2_HexNAc1, and lacto-N-fucopentaose [LNFP]) found in all does. Sialylated MOs predominanted, with a large dominance of sialyllactose (median: 71% of relative abundance). However, 70% of the rabbit MOs were fucosylated. Over the course of lactation, sialyllactose relative abundance decreased, whereas LNFP increased (30% and 45% respectively at day 25 of lactation). MO profiles in the second lactation were weakly related to offspring survival over 7 breeding cycles. At 8 d of age, fucosyllactose positively correlated with Fusicatenibacter in suckling young rabbit cecal contents. At 26 d, the relative abundance of Oscillospiraceae NK4A214 group was positively associated with Hex4_HexNAc1_Fuc1, whereas Ruminococcus, Alistipes, and Coprococcus were negatively associated with sialyllacto-N-fucopentaose, Hex3_HexNAc2_Fuc1 and sialyllacto-N-tetraose, respectively.
CONCLUSIONS: Rabbit MOs are variable among individuals and across lactation stages, with certain MOs linked to early microbiota composition of the offspring, highlighting their potential prebiotic role in neonatal development.
OBJECTIVE: Our aims were as follows 1) to characterize the MOs in rabbit, 2) to assess the variability in MO profiles over the course of lactation, 3) to evaluate associations with young rabbit survival, and 4) to explore links with cecal microbiota composition.
METHODS: MOs from 76 rabbit does were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Changes over lactation were evaluated in a subset of 10 does. Young rabbit survival data were recorded across 7 reproductive cycles in 67 does. Cecal microbiota composition was assessed in 20 young rabbits at 8 and 26 d and correlated with maternal MO profiles.
RESULTS: A total of 49 MOs were identified, with 3 (sialyllactose, Hex2_HexNAc1, and lacto-N-fucopentaose [LNFP]) found in all does. Sialylated MOs predominanted, with a large dominance of sialyllactose (median: 71% of relative abundance). However, 70% of the rabbit MOs were fucosylated. Over the course of lactation, sialyllactose relative abundance decreased, whereas LNFP increased (30% and 45% respectively at day 25 of lactation). MO profiles in the second lactation were weakly related to offspring survival over 7 breeding cycles. At 8 d of age, fucosyllactose positively correlated with Fusicatenibacter in suckling young rabbit cecal contents. At 26 d, the relative abundance of Oscillospiraceae NK4A214 group was positively associated with Hex4_HexNAc1_Fuc1, whereas Ruminococcus, Alistipes, and Coprococcus were negatively associated with sialyllacto-N-fucopentaose, Hex3_HexNAc2_Fuc1 and sialyllacto-N-tetraose, respectively.
CONCLUSIONS: Rabbit MOs are variable among individuals and across lactation stages, with certain MOs linked to early microbiota composition of the offspring, highlighting their potential prebiotic role in neonatal development.