Fertility and sterility · 2009
Prokineticin 1 mRNA expression in the endometrium of healthy women and in the eutopic endometrium of women with endometriosis.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Tiberi Federica, Tropea Anna, Apa Rosanna, Romani Federica, Lanzone Antonio, Marana Riccardo
Аннотация
OBJECTIVE: To examine prokineticin 1 (PROK1) mRNA expression in eutopic endometrial glands obtained from patients with or without endometriosis, to investigate the presence of additional endometrial abnormalities in women with endometriosis.
DESIGN: Prospective laboratory study.
SETTING: University hospital.
PATIENTS: Twelve control women and 12 patients affected by endometriosis in the secretory phase of the menstrual cycle.
INTERVENTION(S): Endometrial specimens were obtained from women affected (cases) or not (control group) by endometriosis. Endometrial glands were freshly isolated from endometrial biopsies.
MAIN OUTCOME MEASURE(S): PROK1 mRNA expression levels by real-time polymerase chain reaction analysis.
RESULTS: PROK1 mRNA was detectable in 4 of 12 (33%) samples obtained from women affected by endometriosis, whereas 10 of 12 (83%) samples obtained from normal women were positive for PROK1 detection by real-time polymerase chain reaction. Moreover, detectable PROK1 mRNA levels were 10 times lower in samples obtained from women with endometriosis than in samples obtained from control women.
CONCLUSION(S): PROK1 is a newly discovered angiogenic factor implicated in the vascular function of peri-implantation endometrium and early pregnancy. An altered expression of PROK1 could be one of the several biochemical abnormalities characterizing eutopic endometrium in endometriosis.
DESIGN: Prospective laboratory study.
SETTING: University hospital.
PATIENTS: Twelve control women and 12 patients affected by endometriosis in the secretory phase of the menstrual cycle.
INTERVENTION(S): Endometrial specimens were obtained from women affected (cases) or not (control group) by endometriosis. Endometrial glands were freshly isolated from endometrial biopsies.
MAIN OUTCOME MEASURE(S): PROK1 mRNA expression levels by real-time polymerase chain reaction analysis.
RESULTS: PROK1 mRNA was detectable in 4 of 12 (33%) samples obtained from women affected by endometriosis, whereas 10 of 12 (83%) samples obtained from normal women were positive for PROK1 detection by real-time polymerase chain reaction. Moreover, detectable PROK1 mRNA levels were 10 times lower in samples obtained from women with endometriosis than in samples obtained from control women.
CONCLUSION(S): PROK1 is a newly discovered angiogenic factor implicated in the vascular function of peri-implantation endometrium and early pregnancy. An altered expression of PROK1 could be one of the several biochemical abnormalities characterizing eutopic endometrium in endometriosis.